| Characterization of a calcitonin gene-related peptide release assay in rat isolated distal colon. | |
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MedLine Citation:
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PMID: 20162407 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The release of calcitonin gene-related peptide (CGRP) plays a key role gastrointestinal tract homeostasis. We aimed to investigate mechanisms that mediate CGRP release from the rat colon in vitro. Colon segments were stimulated and the amount of CGRP released was measured using an enzyme immunoassay. Capsaicin and low pH induced significant increases in CGRP release which was shown to be mediated by TRPV1 activation as demonstrated with the TRPV1 antagonists CTPC and capsazepine. The mast cell degranulator, compound 48/80 significantly increased CGRP release an effect that was blocked in the presence of the mast cell stabilizer, ketotifen and the selective Gi inhibitor benzalkonium chloride. The addition of a mixture of inflammatory mediators containing pro-inflammatory cytokines, 5HT, bradykinin and PGE2 showed no effect at neutral pH but at low pH a significant additive effect was observed. We conclude that CGRP release in the rat distal colon occurs in response to mast cell degranulation, inflammatory mediators, low pH and capsaicin and describe a role for TRPV1 receptors in mediating the response. |
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Authors:
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Rejbinder Kaur; Celestine T O'Shaughnessy; Emma M Jarvie; Wendy J Winchester; Peter G McLean |
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Publication Detail:
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Type: In Vitro; Journal Article Date: 2010-02-17 |
Journal Detail:
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Title: Archives of pharmacal research Volume: 32 ISSN: 0253-6269 ISO Abbreviation: Arch. Pharm. Res. Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2010-02-17 Completed Date: 2010-05-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8000036 Medline TA: Arch Pharm Res Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 1775-81 Citation Subset: IM |
Affiliation:
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GlaxoSmithKline, Medicines Research Centre, Gunnels Wood Road, Stevenage, Hertfordshire, SG1 2NY, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amiloride
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chemistry,
pharmacology Animals Anti-Allergic Agents / pharmacology Benzalkonium Compounds / pharmacology Calcitonin Gene-Related Peptide / chemistry, metabolism* Capsaicin / analogs & derivatives, chemistry, pharmacology Colon / chemistry, metabolism* Hydrogen-Ion Concentration Inflammation Mediators / pharmacology Ketotifen / pharmacology Male Mast Cells / drug effects Nerve Tissue Proteins / antagonists & inhibitors Neurons, Afferent / chemistry, metabolism Rats Rats, Sprague-Dawley Sodium Channels Solutions TRPV Cation Channels / antagonists & inhibitors p-Methoxy-N-methylphenethylamine / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/ASIC channel; 0/Anti-Allergic Agents; 0/Benzalkonium Compounds; 0/Inflammation Mediators; 0/Nerve Tissue Proteins; 0/Sodium Channels; 0/Solutions; 0/TRPV Cation Channels; 0/Trpv1 protein, rat; 0/capsazepine; 2609-46-3/Amiloride; 34580-13-7/Ketotifen; 404-86-4/Capsaicin; 4091-50-3/p-Methoxy-N-methylphenethylamine; 83652-28-2/Calcitonin Gene-Related Peptide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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