Document Detail

Characterization of cDNAs encoding a new family of tetraspanins from schistosomes--the Sj25 family.
MedLine Citation:
PMID:  9756983     Owner:  NLM     Status:  MEDLINE    
The tetraspanins, also known as members of the transmembrane 4 superfamily (TM4SF), comprise an assemblage of surface antigens reported from mammalian and other vertebrate cells, from schistosomes, from fruit flies and from Caenorhabditis elegans. Tetraspanins are characterized by the presence of four hydrophobic domains, which are presumed to be membrane-spanning, and specific conserved motifs. A novel cDNA encoding a new tetraspanin, termed TE736 (tetraspanin 736), was isolated and characterized from the human blood fluke Schistosoma japonicum. Nucleotide and deduced amino acid sequences of the cDNA revealed that TE736 was similar to the previously characterized Sm23/Sj23/Sh23 species homologues and to Sj25/TM4 from schistosomes, and to other tetraspanins. Comparison of hydropathicity profiles of TE736, Sj25/TM4 and two novel tetraspanin-like sequences from S. mansoni showed that they contain four potential transmembrane domains like other tetraspanins. Sequence alignments showed there are four highly conserved, cysteine residues on the second predicted extracellular loop of TE736. A phylogenetic comparison of the relationship of approximately 30 tetraspanins from mammals and other groups revealed that TE736, Sj25/TM4 and the two sequences from S. mansoni formed an independent family of tetraspanins. We have termed these tetraspanins from schistosomes the Sj25 family. TE736 appeared to be encoded by a single gene and to be expressed in at least two life cycle stages of S. japonicum.
J Fan; P J Brindley
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Gene     Volume:  219     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1998-12-02     Completed Date:  1998-12-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  1-8     Citation Subset:  IM    
Molecular Parasitology Unit, and Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research, Post Office, Royal Brisbane Hospital, Herston, Queensland, 4029, Australia.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AA230187;  AA681057;  AA681058;  AF036955
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MeSH Terms
Amino Acid Sequence
Antigens, Helminth / genetics
Base Sequence
Caenorhabditis elegans
DNA, Complementary
Diptera / genetics
Evolution, Molecular
Expressed Sequence Tags
Gene Library
Helminth Proteins / biosynthesis,  chemistry,  genetics*
Membrane Proteins / biosynthesis,  chemistry,  genetics*
Molecular Sequence Data
Multigene Family*
Protein Conformation
Schistosoma japonicum / genetics*
Sequence Alignment
Sequence Homology, Amino Acid
Reg. No./Substance:
0/Antigens, Helminth; 0/DNA, Complementary; 0/Helminth Proteins; 0/Membrane Proteins; 0/Sj25 protein, Schistosoma japonicum

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