Document Detail

Characterization by human autoantibody of a nuclear antigen related to the cell cycle.
MedLine Citation:
PMID:  6357785     Owner:  NLM     Status:  MEDLINE    
Using a serum from a patient with an autoimmune disease, we have recently described a novel 55 000-dalton antigen (p55) in the nucleus of several animal cells including human ones. This antigen, designated PSL, was not related to the previously defined antigens recognized by sera from patients with systemic rheumatic diseases (Sm, n-RNP, SS-B, Scl-70). We have now found that p55 is associated with chromatin structures as it is released from the nucleus of mink cell fibroblasts by saline + DNase treatments. Analysis by sucrose gradient centrifugation of the nuclear material released in these conditions indicated that p55 co-migrated with core histones. Meanwhile, p55 was absent from the residual nuclear matrices (achromatinic nuclei). Localization of p55 in synchronized cells was performed by indirect immunofluorescence and immunoprecipitation. P55 appeared to accumulate in the nucleus during the S phase. Finally, it was not recognized by an anti-SV40 tumor serum that specifically precipitated the protein p53, which has been recently related to cell proliferation. Thus, PSL an p53, although apparently not antigenically related, appear to be implicated in the same step of the cell cycle.
J P Barque; F Danon; L Peraudeau; P Yeni; C J Larsen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The EMBO journal     Volume:  2     ISSN:  0261-4189     ISO Abbreviation:  EMBO J.     Publication Date:  1983  
Date Detail:
Created Date:  1984-01-07     Completed Date:  1984-01-07     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  743-9     Citation Subset:  IM    
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MeSH Terms
Antigens / immunology*
Autoantibodies / analysis*
Autoimmune Diseases / immunology*
Cell Cycle
Centrifugation, Density Gradient
Fluorescent Antibody Technique
Microscopy, Fluorescence
Reg. No./Substance:
0/Antigens; 0/Autoantibodies

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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