Document Detail

Characterization of branched and unsaturated fatty acids in Mycobacterium vaccae strain JOB5.
MedLine Citation:
PMID:  1122427     Owner:  NLM     Status:  MEDLINE    
After growth of Mycobacterium vaccae strain JOB5 on acetate or propane, the cellular fatty acids were isolated and identified by a combination of gas-chromatographic, mass-spectral, and chemical means. The fatty acids ranged from C12 to C19 and were a mixture of saturated, monounsaturated, and methyl-branched components. The double bond was in the delta9 position in the C15 to C18 unsaturated acids. The single methyl branch was located on the C10 position of Br-C17, Br-C18, and Br-C19 fatty acids. Branched-chain synthesis occurs at the expense of an unsaturated precursor fatty acid; the double bond serves as the site of methylation. Results suggest that S-adenosylmethionine is the methyl donor involved.
D H King; J J Perry
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Canadian journal of microbiology     Volume:  21     ISSN:  0008-4166     ISO Abbreviation:  Can. J. Microbiol.     Publication Date:  1975 Apr 
Date Detail:
Created Date:  1975-07-03     Completed Date:  1975-07-03     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0372707     Medline TA:  Can J Microbiol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  510-2     Citation Subset:  IM    
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MeSH Terms
Acetates / metabolism
Carbon Radioisotopes
Chromatography, Gas
Fatty Acids / analysis*,  biosynthesis
Fatty Acids, Unsaturated / analysis*
Methionine / metabolism
Mycobacterium / analysis*,  metabolism
Propane / metabolism
Reg. No./Substance:
0/Acetates; 0/Carbon Radioisotopes; 0/Fatty Acids; 0/Fatty Acids, Unsaturated; 63-68-3/Methionine; 74-98-6/Propane

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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