Document Detail

Characterization of bone cells isolated on discontinuous Percoll gradients: distribution in sequentially derived populations.
MedLine Citation:
PMID:  1665004     Owner:  NLM     Status:  MEDLINE    
Bone cell populations obtained by sequential digestion of newborn mouse calvariae remain morphologically heterogeneous despite well-documented biochemical differences. Fractionation of these populations on Percoll gradient reveal three major cell groups of low, intermediate, and high buoyant density (1.056, 1.070, and 1.095 g/ml) that are present in different ratios in early and late released populations. Cells of low and intermediate density dominate in early released populations. In contrast, late released populations contain mostly high-density cells. Basal levels of alkaline phosphatase are highest in cells of intermediate buoyant density. All cells respond to PTH with cAMP production and morphologic transformation, but biochemical responses to PTH, such as secretion of insulin-like growth factor I (IGF-I) and stimulation of alkaline phosphatase activity, occur mostly in cells of intermediate density. These data suggest that (1) subclasses of osteoblasts can be further separated by density and (2) PTH effects on alkaline phosphatase activity and IGF-I secretion are probably expressed by osteoblasts of a certain subclass and/or stage of development.
G L Wong; M C Ng; D W Calabrese; D E Swartzendruber
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research     Volume:  6     ISSN:  0884-0431     ISO Abbreviation:  J. Bone Miner. Res.     Publication Date:  1991 Sep 
Date Detail:
Created Date:  1992-03-23     Completed Date:  1992-03-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8610640     Medline TA:  J Bone Miner Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  969-76     Citation Subset:  IM    
University of Colorado, Colorado Springs.
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MeSH Terms
Alkaline Phosphatase / metabolism
Binding Sites
Bone and Bones / cytology*
Cell Separation
Cells, Cultured
Centrifugation, Density Gradient
Cyclic AMP / metabolism
DNA / analysis
Epidermal Growth Factor / metabolism
Flow Cytometry
Insulin-Like Growth Factor I / secretion
Osteoblasts / chemistry,  cytology*,  metabolism
Parathyroid Hormone / pharmacology
Grant Support
Reg. No./Substance:
0/Parathyroid Hormone; 60-92-4/Cyclic AMP; 62229-50-9/Epidermal Growth Factor; 67763-96-6/Insulin-Like Growth Factor I; 9007-49-2/DNA; EC Phosphatase

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