Document Detail

Characterization of biotinylated proteins in mammalian cells using 125I-streptavidin.
MedLine Citation:
PMID:  3443696     Owner:  NLM     Status:  MEDLINE    
The presence of biotinylated proteins in detergent extracts of cells is demonstrated using 125I-streptavidin. Four components with apparent molecular masses (Mr) ranging from 70 to 220 kDa have been detected. These probably represent the biotinylated subunits of the carboxylase enzymes. The proportions of the four components were found to vary from cell type to cell type and also the Mr varied from species to species.
M E Bramwell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biochemical and biophysical methods     Volume:  15     ISSN:  0165-022X     ISO Abbreviation:  J. Biochem. Biophys. Methods     Publication Date:  1987 Dec 
Date Detail:
Created Date:  1988-04-27     Completed Date:  1988-04-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7907378     Medline TA:  J Biochem Biophys Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  125-32     Citation Subset:  IM    
Sir William Dunn School of Pathology, Oxford, U.K.
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MeSH Terms
Bacterial Proteins / diagnostic use*
Biotin / analogs & derivatives*,  analysis
Cells, Cultured
Electrophoresis, Polyacrylamide Gel
Iodine Radioisotopes / diagnostic use
Proteins / analysis*
Tumor Cells, Cultured
Reg. No./Substance:
0/Bacterial Proteins; 0/Iodine Radioisotopes; 0/Proteins; 58-85-5/Biotin; 9013-20-1/Streptavidin

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