Document Detail


Characterization of biodegradable poly(D,L-lactide-co-glycolide) nanoparticles loaded with bacteriochlorophyll-a for photodynamic therapy.
MedLine Citation:
PMID:  17975957     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Bacteriochlorophyll-a (BChl-a) was incorporated into nanoparticles of poly(D,L-lactide-co-glycolide) (PLGA), in order to evaluate this photosensitizer when associated with this specific drug delivery system (DDS) to analyze its physical and spectral properties. BACKGROUND DATA: It has been suggested in the literature that BChl-a is a potential sensitizer in photodynamic tumor therapy and until now has only been investigated in organic media. METHODS: Nanoparticles loaded with the photosensitizer BChl-a were prepared by the solvent evaporation technique. Parameters such as particle size, drug encapsulation efficiency, external morphology, and in vitro release behavior were evaluated. The photophysical properties (absorption, fluorescence, fluorescence quantum yields, and singlet oxygen quantum yields) of BChl-a, were investigated in toluene and when encapsulated in nanoparticles. RESULTS: Scanning electron microscopy and dynamic light scattering revealed that particles loaded with BChl-a are spherical in shape, and they have a diameter of 660 nm and a low tendency to aggregate. The encapsulation efficiency obtained in this procedure was 69%. Spectroscopic analysis showed an absorption band centered at 782 nm in nanoparticles used as a DDS. Fluorescence quantum yield (phi(F) = 0.19) and higher efficiency in singlet oxygen production (Phi(Delta) = 0.26) was also observed. CONCLUSIONS: The results suggest that this DDS is potentially useful for the delivery and release of BChl-a as a photosensitizer in photodynamic therapy (PDT) protocols due to its excellent biodegradability, biocompatibility, and maintenance of its spectroscopic properties when compared with other dyes in homogenous media. The biocompatible DDS medium is a new proposal and will make feasible the use of BChl-a as a dye for PDT, improving the utilization of non-organic media to deliver this kind of molecule.
Authors:
Anderson J Gomes; Claure N Lunardi; Antonio C Tedesco
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Photomedicine and laser surgery     Volume:  25     ISSN:  1549-5418     ISO Abbreviation:  -     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-11-02     Completed Date:  2008-02-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101222340     Medline TA:  Photomed Laser Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  428-35     Citation Subset:  IM    
Affiliation:
Faculdade de Filosofia Ciências e Letras de Ribeirão Preto (FFCLRP-USP), Ribeirão Preto, SP, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Bacteriochlorophyll A / administration & dosage,  chemistry*
Biocompatible Materials*
Drug Compounding
Drug Delivery Systems*
Drug Stability
Lactic Acid*
Nanoparticles*
Photochemotherapy
Photosensitizing Agents / administration & dosage,  chemistry*
Polyglycolic Acid*
Polymers*
Chemical
Reg. No./Substance:
0/Bacteriochlorophyll A; 0/Biocompatible Materials; 0/Photosensitizing Agents; 0/Polymers; 0/polylactic acid-polyglycolic acid copolymer; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid

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