Document Detail

Characterization of beta3-adrenoceptors in human internal mammary artery and putative involvement in coronary artery bypass management.
MedLine Citation:
PMID:  16022967     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: The aim of the present study was to analyze whether beta3-adrenoceptors (beta3-ARs) were effectively present and functional in the human internal mammary artery (IMA). BACKGROUND: The beta1- and beta2-adrenoceptors classically mediate the relaxant effects of catecholamines in the vessels. In vitro and in vivo studies performed in various animal species described vasodilating effects due to activation of a third beta-ARs subtype (beta3). METHODS: Reverse transcription-polymerase chain reaction analysis, Western blot experiments, and pharmacological studies were carried out in human IMA samples harvested from 27 patients undergoing coronary bypass surgery. RESULTS: The beta3-ARs messenger ribonucleic acid and protein were detected in intact IMA, but were absent in endothelium-free samples. This finding was confirmed by immunohistochemical experiments. In organ baths, a beta3-AR agonist, SR 58611A, induced an endothelium-dependent relaxation of phenylephrine-precontracted IMA rings. This vasodilation was not modified by beta1/beta2-AR antagonists, but was greatly altered in the presence of L-748,337, a selective human beta3-AR antagonist. Moreover, the inhibition of nitric oxide (NO) synthases abolished the beta3-adrenergic vasodilation, suggesting the involvement of a NO-signaling pathway. CONCLUSIONS: Those results demonstrated the presence of beta3-ARs in the endothelial layer of human IMA. The present work highlights the role of beta3-ARs in vasomotor control of IMA and opens new fields of investigation in coronary bypass graft management, heart failure, and hypertension.
Bertrand Rozec; Sabrina Serpillon; Gilles Toumaniantz; Camille Sèze; Yohann Rautureau; Olivier Baron; Jacques Noireaud; Chantal Gauthier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the American College of Cardiology     Volume:  46     ISSN:  0735-1097     ISO Abbreviation:  J. Am. Coll. Cardiol.     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-07-18     Completed Date:  2005-08-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8301365     Medline TA:  J Am Coll Cardiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  351-9     Citation Subset:  AIM; IM    
L'Institut du Thorax, INSERM UMR533, Faculté de Médecine, Nantes, France.
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MeSH Terms
Adrenergic beta-Agonists / pharmacology
Blotting, Western
Endothelium, Vascular / metabolism
Internal Mammary-Coronary Artery Anastomosis*
Mammary Arteries / cytology,  metabolism*
RNA, Messenger / analysis
Receptors, Adrenergic, beta-3 / metabolism*,  physiology
Reverse Transcriptase Polymerase Chain Reaction
Tetrahydronaphthalenes / pharmacology
Vasomotor System / physiology
Reg. No./Substance:
0/Adrenergic beta-Agonists; 0/RNA, Messenger; 0/Receptors, Adrenergic, beta-3; 0/Tetrahydronaphthalenes; 121524-09-2/SR 58611A

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