| Characterization of beta3-adrenoceptors in human internal mammary artery and putative involvement in coronary artery bypass management. | |
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MedLine Citation:
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PMID: 16022967 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: The aim of the present study was to analyze whether beta3-adrenoceptors (beta3-ARs) were effectively present and functional in the human internal mammary artery (IMA). BACKGROUND: The beta1- and beta2-adrenoceptors classically mediate the relaxant effects of catecholamines in the vessels. In vitro and in vivo studies performed in various animal species described vasodilating effects due to activation of a third beta-ARs subtype (beta3). METHODS: Reverse transcription-polymerase chain reaction analysis, Western blot experiments, and pharmacological studies were carried out in human IMA samples harvested from 27 patients undergoing coronary bypass surgery. RESULTS: The beta3-ARs messenger ribonucleic acid and protein were detected in intact IMA, but were absent in endothelium-free samples. This finding was confirmed by immunohistochemical experiments. In organ baths, a beta3-AR agonist, SR 58611A, induced an endothelium-dependent relaxation of phenylephrine-precontracted IMA rings. This vasodilation was not modified by beta1/beta2-AR antagonists, but was greatly altered in the presence of L-748,337, a selective human beta3-AR antagonist. Moreover, the inhibition of nitric oxide (NO) synthases abolished the beta3-adrenergic vasodilation, suggesting the involvement of a NO-signaling pathway. CONCLUSIONS: Those results demonstrated the presence of beta3-ARs in the endothelial layer of human IMA. The present work highlights the role of beta3-ARs in vasomotor control of IMA and opens new fields of investigation in coronary bypass graft management, heart failure, and hypertension. |
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Authors:
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Bertrand Rozec; Sabrina Serpillon; Gilles Toumaniantz; Camille Sèze; Yohann Rautureau; Olivier Baron; Jacques Noireaud; Chantal Gauthier |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of the American College of Cardiology Volume: 46 ISSN: 0735-1097 ISO Abbreviation: J. Am. Coll. Cardiol. Publication Date: 2005 Jul |
Date Detail:
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Created Date: 2005-07-18 Completed Date: 2005-08-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8301365 Medline TA: J Am Coll Cardiol Country: United States |
Other Details:
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Languages: eng Pagination: 351-9 Citation Subset: AIM; IM |
Affiliation:
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L'Institut du Thorax, INSERM UMR533, Faculté de Médecine, Nantes, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adrenergic beta-Agonists
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pharmacology Aged Animals Blotting, Western Endothelium, Vascular / metabolism Female Humans Immunohistochemistry Internal Mammary-Coronary Artery Anastomosis* Male Mammary Arteries / cytology, metabolism* RNA, Messenger / analysis Receptors, Adrenergic, beta-3 / metabolism*, physiology Reverse Transcriptase Polymerase Chain Reaction Tetrahydronaphthalenes / pharmacology Vasomotor System / physiology |
| Chemical | |
Reg. No./Substance:
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0/Adrenergic beta-Agonists; 0/RNA, Messenger; 0/Receptors, Adrenergic, beta-3; 0/Tetrahydronaphthalenes; 121524-09-2/SR 58611A |
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