Document Detail

Characterization of beta-lactam antagonist in Supplement C.
MedLine Citation:
PMID:  3149566     Owner:  NLM     Status:  MEDLINE    
In the course of using Mueller-Hinton agar with 1% Supplement C (SC) (Difco, Detroit, MI) as a susceptibility test medium for Haemophilus influenzae, one lot of SC was encountered whose use was associated with markedly increased ampicillin MICs. Acidimetric and chromogenic cephalosporin filter paper disc tests of SC failed to detect beta-lactamase activity. Macrobroth dilution MIC tests to determine substrate specificity showed SC to antagonize benzylpenicillin and ampicillin but not cephalothin, cefazolin, or cefaclor, with the antagonism being prevented by the addition of clavulanic acid. High pressure liquid chromatographic analysis of reference and reaction solutions of benzylpenicillin with SC showed almost complete degradation of benzylpenicillin to benzylpenicilloic acid after 24 hr at 37 degrees C. For two other lots of SC that had passed MIC quality control testing, similar high pressure liquid chromatographic studies demonstrated slow conversion of small amounts of benzylpenicillin to benzylpenicilloic acid. These findings indicate that the beta-lactam antagonism by SC was due to the presence of a contaminating beta-lactamase directed primarily toward the penicillins.
B F Woolfrey; M E Gresser-Burns; D Ehresman; R T Lally
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Diagnostic microbiology and infectious disease     Volume:  10     ISSN:  0732-8893     ISO Abbreviation:  Diagn. Microbiol. Infect. Dis.     Publication Date:  1988 Aug 
Date Detail:
Created Date:  1989-05-26     Completed Date:  1989-05-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8305899     Medline TA:  Diagn Microbiol Infect Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  213-9     Citation Subset:  IM    
Department of Anatomic and Clinical Pathology, St. Paul-Ramsey Medical Center, MN 55101.
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MeSH Terms
Anti-Bacterial Agents / antagonists & inhibitors*
Chromatography, High Pressure Liquid
Culture Media*
Drug Contamination
Enterococcus faecalis / drug effects
Haemophilus influenzae / drug effects
Microbial Sensitivity Tests*
beta-Lactamases / analysis
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Culture Media; 0/beta-Lactams; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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