| Characterization of beta-lactam antagonist in Supplement C. | |
| | |
MedLine Citation:
|
PMID: 3149566 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In the course of using Mueller-Hinton agar with 1% Supplement C (SC) (Difco, Detroit, MI) as a susceptibility test medium for Haemophilus influenzae, one lot of SC was encountered whose use was associated with markedly increased ampicillin MICs. Acidimetric and chromogenic cephalosporin filter paper disc tests of SC failed to detect beta-lactamase activity. Macrobroth dilution MIC tests to determine substrate specificity showed SC to antagonize benzylpenicillin and ampicillin but not cephalothin, cefazolin, or cefaclor, with the antagonism being prevented by the addition of clavulanic acid. High pressure liquid chromatographic analysis of reference and reaction solutions of benzylpenicillin with SC showed almost complete degradation of benzylpenicillin to benzylpenicilloic acid after 24 hr at 37 degrees C. For two other lots of SC that had passed MIC quality control testing, similar high pressure liquid chromatographic studies demonstrated slow conversion of small amounts of benzylpenicillin to benzylpenicilloic acid. These findings indicate that the beta-lactam antagonism by SC was due to the presence of a contaminating beta-lactamase directed primarily toward the penicillins. |
| | |
Authors:
|
B F Woolfrey; M E Gresser-Burns; D Ehresman; R T Lally |
Related Documents
:
|
8286416 - Effects of double amino-acid substitution polymorphism in acid beta-galactosidase gene ... 15934776 - Reactions of pyrazolylborate-zinc-hydroxide complexes related to beta-lactamase activity. 14671486 - Dietary dunaliella bardawil, a beta-carotene-rich alga, protects against acetic acid-in... 19449346 - Kinetic resolution of aliphatic beta-amino acid amides by beta-aminopeptidases. 22381886 - Field enhancement sample stacking for analysis of organic acids in traditional chinese ... 18825276 - Significant associations of stearoyl-coa desaturase (scd1) gene with fat deposition and... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Diagnostic microbiology and infectious disease Volume: 10 ISSN: 0732-8893 ISO Abbreviation: Diagn. Microbiol. Infect. Dis. Publication Date: 1988 Aug |
Date Detail:
|
Created Date: 1989-05-26 Completed Date: 1989-05-26 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8305899 Medline TA: Diagn Microbiol Infect Dis Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 213-9 Citation Subset: IM |
Affiliation:
|
Department of Anatomic and Clinical Pathology, St. Paul-Ramsey Medical Center, MN 55101. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Anti-Bacterial Agents
/
antagonists & inhibitors* Chromatography, High Pressure Liquid Culture Media* Drug Contamination Enterococcus faecalis / drug effects Haemophilus influenzae / drug effects Microbial Sensitivity Tests* beta-Lactamases / analysis beta-Lactams |
| Chemical | |
Reg. No./Substance:
|
0/Anti-Bacterial Agents; 0/Culture Media; 0/beta-Lactams; EC 3.5.2.6/beta-Lactamases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Clinical pharmacokinetics of valproic acid--1988.
Next Document: In vitro activity of fleroxacin (Ro23-6240), a new fluorinated 4-quinolone against isolates from can...