Document Detail


Characterization of beta-adrenergic receptors in cultured human trabecular cells and in human trabecular meshwork.
MedLine Citation:
PMID:  2536360     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The characterization of beta-adrenergic receptors on cultured human trabecular cells and trabecular meshwork from human autopsy eyes was carried out by radioligand binding utilizing (125I)-iodopindolol. In cultured cells, the observed binding of (125I)-iodopindolol was of high affinity (Kd = 43 pM) and saturable. Scatchard plots were linear and revealed a Bmax of 33 +/- 7 fmol/mg of protein. Competition studies with a series of agonists and antagonists revealed that human trabecular cells contain a single class of beta-adrenergic receptors of the beta 2 subtype. Similarly, the IC50 of ICI 89,406 (176 nM) in human trabecular meshwork from autopsy eyes supports the presence of beta 2-adrenergic receptors in this tissue.
Authors:
M B Wax; P B Molinoff; J Alvarado; J Polansky
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  30     ISSN:  0146-0404     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  1989 Jan 
Date Detail:
Created Date:  1989-02-28     Completed Date:  1989-02-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  51-7     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104-6084.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Binding Sites
Cells, Cultured
Humans
Middle Aged
Pindolol / analogs & derivatives,  metabolism
Receptors, Adrenergic, beta / metabolism*
Trabecular Meshwork / cytology,  metabolism*
Grant Support
ID/Acronym/Agency:
EY-06810/EY/NEI NIH HHS; MH14654/MH/NIMH NIH HHS; NS 18479/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Receptors, Adrenergic, beta; 13523-86-9/Pindolol; 76875-01-9/3-iodopindolol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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