Document Detail


Characterization of a basic phospholipase A2-homologeu myotoxin isolated from the venom of the snake Bothrops neuwiedii (yarará chica) from Argentina.
MedLine Citation:
PMID:  10519651     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A basic protein was isolated by CM-Sephadex C-25 chromatography from the venom of Bothrops neuwiedii from Argentina, and named B. neuwiedii myotoxin I. This protein exerted local myotoxic and edema-forming effects in mice, with potencies comparable to other myotoxins isolated from Bothrops spp. venoms. When injected by i.v. route at doses up to 4.7 mg/kg of body weight, the toxin was not lethal. In vitro, the toxin had no detectable phospholipase A2 activity on egg yolk phospholipids. B. neuwiedii myotoxin I appeared as a homodimer in sodium dodecylsulphate-polyacrylamide gel electrophoresis, with a subunit molecular weight of 15 kD. Gel immunodiffusion revealed a pattern of partial antigenic identity between the newly isolated myotoxin and myotoxin II from Bothrops asper venom. The sequence of B. neuwiedii myotoxin I was determined for the first 40 amino acid residues, showing high homology to several class II phospholipase A2 myotoxins of the Lys-49 family from crotalids. Altogether, results suggest that this toxin is a new member of the Lys-49 phospholipase A2-homologues with myotoxic, cytolytic, and edema-inducing activities.
Authors:
P Geoghegan; Y Angulo; A Cangelosi; M Díaz; B Lomonte
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  37     ISSN:  0041-0101     ISO Abbreviation:  Toxicon     Publication Date:  1999 Dec 
Date Detail:
Created Date:  1999-10-28     Completed Date:  1999-10-28     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1735-46     Citation Subset:  IM    
Affiliation:
Servicio de Inmunoterapéuticos, CNCCB-ANLIS Carlos G. Malbrán, Buenos Aires, Argentina.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Argentina
Bothrops*
Chromatography, Ion Exchange
Crotalid Venoms / enzymology*
Edema / chemically induced
Electrophoresis, Polyacrylamide Gel
Hemorrhage / chemically induced
Immunodiffusion
Mice
Molecular Sequence Data
Muscle, Skeletal / drug effects*
Muscular Diseases / chemically induced
Phospholipases A / analysis,  isolation & purification*,  pharmacology*
Phospholipases A2
Survival Rate
Chemical
Reg. No./Substance:
0/Crotalid Venoms; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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