Document Detail


Characterization of basal pseudopod-like processes in ileal and colonic PYY cells.
MedLine Citation:
PMID:  21061049     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The peptide tyrosine tyrosine (PYY) is produced and secreted from L cells of the gastrointestinal mucosa. To study the anatomy and function of PYY-secreting L cells, we developed a transgenic PYY-green fluorescent protein mouse model. PYY-containing cells exhibited green fluorescence under UV light and were immunoreactive to antibodies against PYY and GLP-1 (glucagon-like peptide-1, an incretin hormone also secreted by L cells). PYY-GFP cells from 15 μm thick sections were imaged using confocal laser scanning microscopy and three-dimensionally (3D) reconstructed. Results revealed unique details of the anatomical differences between ileal and colonic PYY-GFP cells. In ileal villi, the apical portion of PYY cells makes minimal contact with the lumen of the gut. Long pseudopod-like basal processes extend from these cells and form an interface between the mucosal epithelium and the lamina propria. Some basal processes are up to 50 μm in length. Multiple processes can be seen protruding from one cell and these often have a terminus resembling a synapse that appears to interact with neighboring cells. In colonic crypts, PYY-GFP cells adopt a spindle-like shape and weave in between epithelial cells, while maintaining contact with the lumen and lamina propria. In both tissues, cytoplasmic granules containing the hormones PYY and GLP-1 are confined to the base of the cell, often filling the basal process. The anatomical arrangement of these structures suggests a dual function as a dock for receptors to survey absorbed nutrients and as a launching platform for hormone secretion in a paracrine fashion.
Authors:
Diego V Bohórquez; Rashmi Chandra; Leigh Ann Samsa; Steven R Vigna; Rodger A Liddle
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of molecular histology     Volume:  42     ISSN:  1567-2387     ISO Abbreviation:  J. Mol. Histol.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-05-09     Completed Date:  2011-07-15     Revised Date:  2014-06-24    
Medline Journal Info:
Nlm Unique ID:  101193653     Medline TA:  J Mol Histol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  3-13     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Colon / cytology*,  metabolism
Enteroendocrine Cells / cytology
Glucagon-Like Peptide 1 / metabolism
Green Fluorescent Proteins / genetics,  metabolism
Ileum / cytology*,  metabolism
Intestinal Mucosa / cytology,  metabolism
Mice
Mice, Transgenic
Peptide YY / genetics,  metabolism*
Protein Transport / physiology
Pseudopodia / metabolism*,  ultrastructure
Grant Support
ID/Acronym/Agency:
DK38626/DK/NIDDK NIH HHS; R01 DK091946/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
106388-42-5/Peptide YY; 147336-22-9/Green Fluorescent Proteins; 89750-14-1/Glucagon-Like Peptide 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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