Document Detail

Characterization of bacterial and fungal biofilms in chronic rhinosinusitis.
MedLine Citation:
PMID:  19958600     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Conclusive evidence exists that biofilms are present on the mucosa of chronic rhinosinusitis (CRS) patients. Less is known about the species constituting these biofilms. This study developed a fluorescence in situ hybridization (FISH) protocol for characterization of bacterial and fungal biofilms in CRS.
METHODS: Fifty CRS patients and 10 controls were recruited. Bacteria FISH probes for Staphylococcus aureus, Haemophilus influenzae, and Pseudomonas aeruginosa and a universal probe for fungi were applied to sinus mucosal specimens and then analyzed using confocal scanning laser microscopy.
RESULTS: Thirty-six of 50 CRS patients had biofilms present in contrast to 0/10 controls, suggesting a role for biofilms in the pathogenesis of this disease. S. aureus was the most common biofilm-forming organism. Eleven of 50 CRS patients had characteristic fungal biofilms present.
CONCLUSION: This is the largest study of biofilms in CRS. It has validated mucosal tissue cryopreservation for delayed biofilm analysis. Fungal biofilms have been identified and the importance of S. aureus biofilms in the polymicrobial etiology of CRS is highlighted.
Andrew Foreman; Alkis James Psaltis; Lor Wai Tan; Peter-John Wormald
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of rhinology & allergy     Volume:  23     ISSN:  1945-8932     ISO Abbreviation:  Am J Rhinol Allergy     Publication Date:    2009 Nov-Dec
Date Detail:
Created Date:  2009-12-04     Completed Date:  2010-06-09     Revised Date:  2011-11-10    
Medline Journal Info:
Nlm Unique ID:  101490775     Medline TA:  Am J Rhinol Allergy     Country:  United States    
Other Details:
Languages:  eng     Pagination:  556-61     Citation Subset:  IM    
Department of Surgery-Otorhinolaryngology, Head and Neck Surgery, University of Adelaide, Adelaide, Australia.
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MeSH Terms
Chronic Disease
Fungi / growth & development*,  pathogenicity
In Situ Hybridization, Fluorescence
Middle Aged
Mycoses / complications,  diagnosis*
Nasal Mucosa / microbiology*
Rhinitis / etiology,  prevention & control
Sinusitis / etiology,  prevention & control
Staphylococcal Infections / complications,  diagnosis*
Staphylococcus aureus / growth & development*,  pathogenicity

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