Document Detail


Characterization of an autoinducer of penicillin biosynthesis in Penicillium chrysogenum.
MedLine Citation:
PMID:  21724894     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Filamentous fungi produce an impressive variety of secondary metabolites; many of them have important biological activities. The biosynthesis of these secondary metabolites is frequently induced by plant-derived external elicitors and appears to also be regulated by internal inducers, which may work in a way similar to that of bacterial autoinducers. The biosynthesis of penicillin in Penicillium chrysogenum is an excellent model for studying the molecular mechanisms of control of gene expression due to a good knowledge of the biochemistry and molecular genetics of β-lactam antibiotics and to the availability of its genome sequence and proteome. In this work, we first developed a plate bioassay that allows direct testing of inducers of penicillin biosynthesis using single colonies of P. chrysogenum. Using this bioassay, we have found an inducer substance in the conditioned culture broths of P. chrysogenum and Acremonium chrysogenum. No inducing effect was exerted by γ-butyrolactones, jasmonic acid, or the penicillin precursor δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine. The conditioned broth induced penicillin biosynthesis and transcription of the pcbAB, pcbC, and penDE genes when added at inoculation time, but its effect was smaller if added at 12 h and it had no effect when added at 24 h, as shown by Northern analysis and lacZ reporter studies. The inducer molecule was purified and identified by mass spectrometry (MS) and nuclear magnetic resonance (NMR) as 1,3-diaminopropane. Addition of pure 1,3-diaminopropane stimulated the production of penicillin by about 100% compared to results for the control cultures. Genes for the biosynthesis of 1,3-diaminopropane have been identified in the P. chrysogenum genome.
Authors:
Jorge Martín; Carlos García-Estrada; Angel Rumbero; Eliseo Recio; Silvia M Albillos; Ricardo V Ullán; Juan-Francisco Martín
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-07-01
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  77     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-08-05     Completed Date:  2011-12-15     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5688-96     Citation Subset:  IM    
Affiliation:
INBIOTEC, Instituto de Biotecnología de León, Leon, Spain.
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MeSH Terms
Descriptor/Qualifier:
Acremonium / metabolism
Biological Assay / methods
Candida / chemistry
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Culture Media, Conditioned / chemistry
Diamines / isolation & purification,  metabolism*,  pharmacology
Gene Expression Regulation, Fungal
Genes, Fungal
Magnetic Resonance Spectroscopy
Mass Spectrometry
Mycelium / genetics
Penicillins / biosynthesis*
Penicillium chrysogenum / drug effects,  genetics,  metabolism*
Transcription, Genetic
Chemical
Reg. No./Substance:
0/Culture Media, Conditioned; 0/Diamines; 0/Penicillins; 109-76-2/trimethylenediamine
Comments/Corrections

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