Document Detail

Characterization of atherosclerotic plaque depositions by Raman and FTIR imaging.
MedLine Citation:
PMID:  23139154     Owner:  NLM     Status:  Publisher    
Spectroscopy-based imaging techniques can provide useful biochemical information about tissue samples. Here, we employ Raman and Fourier transform infrared (IR) imaging to characterize composition and constitution of atherosclerotic plaques of rabbits, fed with a high cholesterol diet. The results were compared with conventional light microscopy after staining with hematoxylin eosin, and elastica van Gieson. The spectral unmixing algorithm vertex component analysis was applied for data analysis and image reconstruction. IR microscopy allowed for differentiation between lipids and proteins in plaques of full aortic cross sections. Raman microscopy further discriminated cholesterol esters, cholesterol and triglycerides. FTIR and Raman images were recorded at a resolution near 20 micrometer per pixel for a large field of view. High resolution Raman images at 1 micrometer per pixel revealed structural details at selected regions of interest. The intima-media and the lipid-protein ratio were determined in five specimens for quantitation. These results correlate well with histopathology. The described method is a promising tool for easy and fast molecular imaging of atherosclerosis. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim).
Annika Lattermann; Christian Matthäus; Norbert Bergner; Claudia Beleites; Bernd F Romeike; Christoph Krafft; Bernhard R Brehm; Jürgen Popp
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-9
Journal Detail:
Title:  Journal of biophotonics     Volume:  -     ISSN:  1864-0648     ISO Abbreviation:  J Biophotonics     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101318567     Medline TA:  J Biophotonics     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
University Hospital - Friedrich-Schiller-University of Jena, Clinic for Internal Medicine I Erlanger Allee 101, 07747 Jena, Germany; Institute of Photonic Technology, Albert Einstein Straße 9, 07745 Jena, Germany.
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