Document Detail


Characterization of arginine vasopressin actions in human uterine artery: lack of role of the vascular endothelium.
MedLine Citation:
PMID:  7582559     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. The effect of arginine vasopressin (AVP) on human uterine artery rings, both intact and denuded of endothelium, was investigated. 2. Initially, AVP (63 pM-32 nM) induced concentration-dependent contraction of human uterine artery (pD2 = 8.92 +/- 0.01). Removal of the endothelium did not affect the concentration-response curve for AVP (pD2 = 8.83 +/- 0.03). 3. In contrast, human uterine arteries, both intact and denuded of endothelium, did not respond to the addition of 1-desamino-8-D-arginine vasopressin (dDAVP, 1 nM-1 microM). 4. In both types of preparations, [d(CH2)5Tyr(Me)AVP (1-10 nM) and [d(CH2)5,D-Ile2,Ile4]AVP (300 nM-3 microM) produced parallel rightward shifts of the curves for AVP. The Schild plots constrained to a slope of unity gave the following -log KB values: [d(CH2)5Tyr(Me)]AVP vs. [d(CH2)5,D-Ile2,Ile4]AVP 9.66 vs. 6.69 and 9.61 vs. 6.80 for human uterine artery, intact and denuded of endothelium, respectively. 5. The pKA values for AVP itself also did not differ between preparations: 6.56 and 6.43 for human uterine artery with and without endothelium, respectively. In both types of preparations, the receptor reserve (KA/EC50) was considerably greater than unity (intact vs. denuded: 228 vs. 244). 6. It is concluded that, in human uterine artery, AVP induces contractions that are not modulated by the endothelium. It is likely that AVP acts as a full agonist on human uterine artery, regardless of the endothelial condition. On the basis of differential antagonists affinity and affinity of AVP itself, it is probable that vasopressin receptors involved in AVP-induced contraction in human uterine arteries belong to the V1a or V1a-like subtype.
Authors:
A Jovanović; L Grbović; I Zikić; I Tulic
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British journal of pharmacology     Volume:  115     ISSN:  0007-1188     ISO Abbreviation:  Br. J. Pharmacol.     Publication Date:  1995 Aug 
Date Detail:
Created Date:  1995-12-01     Completed Date:  1995-12-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7502536     Medline TA:  Br J Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1295-301     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Medical Faculty, Belgrade, Yugoslavia.
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MeSH Terms
Descriptor/Qualifier:
Adult
Arginine Vasopressin / analogs & derivatives,  pharmacology*
Arteries / drug effects*,  physiology
Endothelium, Vascular / drug effects*,  physiology
Female
Humans
Middle Aged
Receptors, Vasopressin / antagonists & inhibitors
Uterus / blood supply*
Chemical
Reg. No./Substance:
0/Receptors, Vasopressin; 113-79-1/Arginine Vasopressin
Comments/Corrections

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