Document Detail

Characterization of arginine vasopressin actions in human uterine artery: lack of role of the vascular endothelium.
MedLine Citation:
PMID:  7582559     Owner:  NLM     Status:  MEDLINE    
1. The effect of arginine vasopressin (AVP) on human uterine artery rings, both intact and denuded of endothelium, was investigated. 2. Initially, AVP (63 pM-32 nM) induced concentration-dependent contraction of human uterine artery (pD2 = 8.92 +/- 0.01). Removal of the endothelium did not affect the concentration-response curve for AVP (pD2 = 8.83 +/- 0.03). 3. In contrast, human uterine arteries, both intact and denuded of endothelium, did not respond to the addition of 1-desamino-8-D-arginine vasopressin (dDAVP, 1 nM-1 microM). 4. In both types of preparations, [d(CH2)5Tyr(Me)AVP (1-10 nM) and [d(CH2)5,D-Ile2,Ile4]AVP (300 nM-3 microM) produced parallel rightward shifts of the curves for AVP. The Schild plots constrained to a slope of unity gave the following -log KB values: [d(CH2)5Tyr(Me)]AVP vs. [d(CH2)5,D-Ile2,Ile4]AVP 9.66 vs. 6.69 and 9.61 vs. 6.80 for human uterine artery, intact and denuded of endothelium, respectively. 5. The pKA values for AVP itself also did not differ between preparations: 6.56 and 6.43 for human uterine artery with and without endothelium, respectively. In both types of preparations, the receptor reserve (KA/EC50) was considerably greater than unity (intact vs. denuded: 228 vs. 244). 6. It is concluded that, in human uterine artery, AVP induces contractions that are not modulated by the endothelium. It is likely that AVP acts as a full agonist on human uterine artery, regardless of the endothelial condition. On the basis of differential antagonists affinity and affinity of AVP itself, it is probable that vasopressin receptors involved in AVP-induced contraction in human uterine arteries belong to the V1a or V1a-like subtype.
A Jovanović; L Grbović; I Zikić; I Tulic
Related Documents :
21375779 - A double shunt technique for the prevention of ischaemia of a congenital, solitary, pel...
21320709 - Prognostic implications of pulmonary hypertension in patients with severe aortic stenosis.
16825079 - Uterine artery embolization and hysteroscopic resection to treat retained placenta accr...
21611029 - Helical ct angiography of fenestrated stent grafting of abdominal aortic aneurysms.
2960239 - Umbilical artery flow velocity waveforms and the placental vascular bed.
21120809 - Arterial supply of human and bovine testes: a topographic and morphometric comparison s...
21381609 - Size of radial and ulnar artery in local population.
20884229 - Sharp recanalization using a subintimal reentry device, angioplasty, and stent placemen...
15891159 - Vascular occlusion sites determine differences in lesion growth from early apparent dif...
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British journal of pharmacology     Volume:  115     ISSN:  0007-1188     ISO Abbreviation:  Br. J. Pharmacol.     Publication Date:  1995 Aug 
Date Detail:
Created Date:  1995-12-01     Completed Date:  1995-12-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7502536     Medline TA:  Br J Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1295-301     Citation Subset:  IM    
Department of Pharmacology, Medical Faculty, Belgrade, Yugoslavia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Arginine Vasopressin / analogs & derivatives,  pharmacology*
Arteries / drug effects*,  physiology
Endothelium, Vascular / drug effects*,  physiology
Middle Aged
Receptors, Vasopressin / antagonists & inhibitors
Uterus / blood supply*
Reg. No./Substance:
0/Receptors, Vasopressin; 113-79-1/Arginine Vasopressin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Calcitonin gene-related peptide (CGRP)-enhanced non-adrenergic non-cholinergic contraction of guinea...
Next Document:  Enhancing and inhibitory effects of nitric oxide on superoxide anion generation in human polymorphon...