Document Detail


Characterization and application of calcium-dependent beta-propeller phytase from Bacillus amyloliquefaciens DS11.
MedLine Citation:
PMID:  22775008     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The enzyme phytase has broad biotechnological applications, especially in the reduction of phytate, anti-nutritional factors that chelate essential minerals, in human and animal food. We investigated the enzymatic properties of β-propeller phytase (BPP) from Bacillus amyloliquefaciens DS11. Thermal refolding analysis demonstrated that BPP can remarkably restore its enzymatic activity in the presence of 5 mM Ca(2+) to 87% of its original activity after heating to 100°C and subsequent cooling, indicating that the enzyme requires Ca(2+) for appropriate refolding. Furthermore, pH-dependent kinetic studies showed that BPP required excess Ca(2+) for its enzymatic activity as the pH decreased, suggesting that the optimal Ca(2+)-phytate ratio for enzymatic catalysis depends on the pH value of the environment. Finally, we verified the practical application of BPP at two different pHs using soybean meal as a natural source of phytate. Compared to a commercial phytase, BPP efficiently hydrolyzed food phytate over neutral pH ranges.
Authors:
Jae-Hoon Shim; Byung-Chul Oh
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-9
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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