| Characterization of antisense RNA-mediated inhibition of SIV replication. | |
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MedLine Citation:
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PMID: 8699163 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Antisense RNA-mediated inhibition of HIV showed mixed success in previous experiments. In order to elucidate the parameters influencing the efficacy of an antisense RNA approach, retroviral vectors encoding 4.5 kb, 3.5 kb, or 2.5 kb antisense RNA of the gag-pol region of SIV (simian immunodeficiency virus) were constructed and used to transduce a CD4-positive CEM174 cell line. The growth rate of transduced cells was measured, and results showed that antisense RNAs have no detrimental effect on cell growth. Similar levels of antisense RNA expression were observed in all transduced cells by Northern analysis. The transduced cells were challenged with uncloned SIVmac239 at a m.o.i. (multiplicity of infection) of 1.0, 0.1, or 0.01. At a m.o.i. of 1.0 or 0.1, no significant inhibition of viral replication was observed in any antisense construct transduced cells up to 9 days postinfection. At a lower m.o.i. (0.01), viral replication was effectively inhibited in 3.5 kb antisense transduced cells as compared to 4.5 kb and 2.5 kb antisense transduced cells at 15 days postinfection. These data suggest that the size of antisense RNA and the challenge dose play a significant role in achieving effective inhibition. |
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Authors:
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F Y Tung; M H Tung |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of medical virology Volume: 48 ISSN: 0146-6615 ISO Abbreviation: J. Med. Virol. Publication Date: 1996 Apr |
Date Detail:
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Created Date: 1996-09-05 Completed Date: 1996-09-05 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7705876 Medline TA: J Med Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 321-5 Citation Subset: IM; X |
Affiliation:
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Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, PA 15261, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antiviral Agents
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pharmacology* Base Sequence Blotting, Northern Cell Line Cell Transformation, Viral / genetics DNA, Viral / analysis Genetic Vectors / genetics Molecular Sequence Data Proviruses / genetics RNA, Antisense / pharmacology* RNA, Viral* Retroviridae / genetics Simian immunodeficiency virus / drug effects, genetics, physiology* Viral Proteins / analysis Virus Replication / drug effects*, genetics |
| Grant Support | |
ID/Acronym/Agency:
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AI31918/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antiviral Agents; 0/DNA, Viral; 0/RNA, Antisense; 0/RNA, Viral; 0/Viral Proteins |
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