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Characterization and antioxidant activities of an acidic polysaccharide from Camellia sinensis.
MedLine Citation:
PMID:  22210484     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
An acidic polysaccharide from the green tea leaves (Camellia sinensis) beyond the useful date was isolated and characterized. Crude polysaccharides (TPS) was extracted with hot water and separated on a DEAE Sepharose FF gel filtration column to obtain ATPS1. The structure of ATPS1 was characterized by HPGPC, IR, UV, (1)H NMR, and (13)C NMR methods. The molecular weight of ATPS1 was determined as 56kDa by HPGPC. The main glycosyl residues of ATPS1 were α-, β-configuration, including signal of β-d-Galp, α-l-Araf, α-d-Manp, α-Rhap, and α-d-Glcp. The DPPH and hydroxyl radical scavenging activities of ATPS1 are lower than those of crude TPS. However, very low concentration of EGCG significantly enhanced DPPH radical scavenging potential of ATPS1. The reducing power of crude TPS is much higher than that of ATPS1. The reducing power of ATPS1 is very weak; however, very low concentration of EGCG (8.0μg/mL) significantly enhanced the reducing power of ATPS1.
Authors:
Jianbo Xiao; Jianglei Huo; Huixian Jiang; Xinlin Wei; Yuanfeng Wang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-21
Journal Detail:
Title:  International journal of biological macromolecules     Volume:  -     ISSN:  1879-0003     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2012-1-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7909578     Medline TA:  Int J Biol Macromol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier B.V.
Affiliation:
College of Life & Environment Science, Shanghai Normal University, 100 Guilin Rd, Shanghai 200234, PR China; Department of Nutrition, Faculty of Health and Welfare, Okayama Prefectural University, Soja, Okayama, Japan.
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