Document Detail

Characterization of anti-complement compounds from Azadirachta indica.
MedLine Citation:
PMID:  2615417     Owner:  NLM     Status:  MEDLINE    
The crude aqueous extract of Azadirachta indica bark possesses an inhibitory activity on both classical (CP) and alternative pathway (AP) activation of human complement. Purification of the compounds with the guidance of the AP-inhibitory activity involved extraction with methanol, dialysis, ion-exchange procedures and gel-permeation chromatography. This sequence yielded two polymers, NB-I and NB-II, one a highly active compound with a relatively low molecular weight (NB-II) and the other a less active compound with a high molecular weight (NB-I). The polymers were characterized by using colour reactions, TLC, GLC and HPLC after hydrolysis and gel-permeation chromatography as peptidoglycans. The carbohydrate part consisted predominantly of glucose. Arabinose, galactose and mannose were present in minor amounts (NB-II) or only as traces (NB-I). Protein was present for 5.5% in NB-I and for 9.8% in NB-II.
J M van der Nat; L A Hart; W G van der Sluis; H van Dijk; A J van den Berg; K T de Silva; R P Labadie
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of ethnopharmacology     Volume:  27     ISSN:  0378-8741     ISO Abbreviation:  J Ethnopharmacol     Publication Date:  1989 Nov 
Date Detail:
Created Date:  1990-03-07     Completed Date:  1990-03-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7903310     Medline TA:  J Ethnopharmacol     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  15-24     Citation Subset:  IM    
Faculty of Pharmacy, Medical Faculty, State University of Utrecht, The Netherlands.
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MeSH Terms
Chemical Phenomena
Chromatography, Gas
Chromatography, Gel
Chromatography, Ion Exchange
Chromatography, Thin Layer
Complement Inactivator Proteins*
Monosaccharides / analysis
Plant Extracts / analysis,  pharmacology
Plant Proteins / pharmacology
Plants, Medicinal / chemistry*
Sri Lanka
Reg. No./Substance:
0/Complement Inactivator Proteins; 0/Monosaccharides; 0/Plant Extracts; 0/Plant Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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