| Characterization of anti-complement compounds from Azadirachta indica. | |
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MedLine Citation:
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PMID: 2615417 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The crude aqueous extract of Azadirachta indica bark possesses an inhibitory activity on both classical (CP) and alternative pathway (AP) activation of human complement. Purification of the compounds with the guidance of the AP-inhibitory activity involved extraction with methanol, dialysis, ion-exchange procedures and gel-permeation chromatography. This sequence yielded two polymers, NB-I and NB-II, one a highly active compound with a relatively low molecular weight (NB-II) and the other a less active compound with a high molecular weight (NB-I). The polymers were characterized by using colour reactions, TLC, GLC and HPLC after hydrolysis and gel-permeation chromatography as peptidoglycans. The carbohydrate part consisted predominantly of glucose. Arabinose, galactose and mannose were present in minor amounts (NB-II) or only as traces (NB-I). Protein was present for 5.5% in NB-I and for 9.8% in NB-II. |
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Authors:
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J M van der Nat; L A Hart; W G van der Sluis; H van Dijk; A J van den Berg; K T de Silva; R P Labadie |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of ethnopharmacology Volume: 27 ISSN: 0378-8741 ISO Abbreviation: J Ethnopharmacol Publication Date: 1989 Nov |
Date Detail:
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Created Date: 1990-03-07 Completed Date: 1990-03-07 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 7903310 Medline TA: J Ethnopharmacol Country: IRELAND |
Other Details:
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Languages: eng Pagination: 15-24 Citation Subset: IM |
Affiliation:
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Faculty of Pharmacy, Medical Faculty, State University of Utrecht, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chemical Phenomena Chemistry Chromatography, Gas Chromatography, Gel Chromatography, Ion Exchange Chromatography, Thin Layer Complement Inactivator Proteins* Dialysis India Monosaccharides / analysis Plant Extracts / analysis, pharmacology Plant Proteins / pharmacology Plants, Medicinal / chemistry* Sri Lanka |
| Chemical | |
Reg. No./Substance:
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0/Complement Inactivator Proteins; 0/Monosaccharides; 0/Plant Extracts; 0/Plant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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