Document Detail


Characterization of the androgen receptor in the skeletal muscle of the rat.
MedLine Citation:
PMID:  973239     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Skeletal muscle homogenate of prepuberal, adult uncastrated or short-(1-4days) or long-term (20days) castrated male and female rats was incubated at 0degreesC iwth highly labeled (3H)5alpha-dihydrotestosterone (1) or (3H) testosterone. labeled (3H)5alpha-dihydrotestosterone (1) or 3H) testosterone. After incubation bound and free hormone in the 100,000 g cytosol were separated by agargel electrophoresis at low temperature. Furthermore, the percentage distribution of the main metabolites found in the cytosol was analyzed by thin-layer chromatography. Two androgen binding proteins could be found: One with high affinity (apparent dissociation and constant (Kd)= 1.4 - 6.4X10(-9)M) low capacity (receptor), the other with relative low affinity high capacity. The physico-chemical characteristics of the androgen receptor in the rat skeletal muscle cytosol are similar if not identical to those which have been described for the androgen receptor in the prostate and other androgen dependent organs. However, the amount of available 5alpha-DHT-binding sites in the skeletal muscle is about 60 times and 7 times lower than in the prostate and bulbocavernosus/levator ani muscle, respectively. No essential androgen metabolism occurs in vitro at 0degreesC in the skeletal muscle.
Authors:
M Krieg
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Steroids     Volume:  28     ISSN:  0039-128X     ISO Abbreviation:  Steroids     Publication Date:  1976 Aug 
Date Detail:
Created Date:  1976-12-03     Completed Date:  1976-12-03     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0404536     Medline TA:  Steroids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  261-74     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Binding, Competitive
Castration
Cytosol / metabolism
Dihydrotestosterone / metabolism
Female
Kinetics
Male
Muscles / metabolism*
Rats
Sex Factors
Testosterone / metabolism
Chemical
Reg. No./Substance:
521-18-6/Dihydrotestosterone; 58-22-0/Testosterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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