Document Detail

Characterization and amino-terminal sequence of apolipoprotein AI from plasma high density lipoproteins in the preruminant calf, Bos spp.
MedLine Citation:
PMID:  2105728     Owner:  NLM     Status:  MEDLINE    
The major apolipoprotein of calf plasma high-density lipoproteins, apo-AI, has been isolated and characterized. Apolipoprotein AI (apo-AI) was separated from the protein moiety of high-density lipoproteins (d 1.090-1.180 g/ml) by preparative electrophoresis in SDS-polyacrylamide gels followed by electrophoretic elution. Purified calf apo-AI had an Mr of approx. 27,000-28,000 in SDS-polyacrylamide gels, resembling human apo-AI. The amino acid composition of calf apo-AI displayed an overall similarity to that of its human and other mammalian counterparts (baboon, dog, badger, rabbit, rat and mouse), but differed in having higher proportions of glutamic acid, alanine and isoleucine. Amino-terminal amino acid sequence analysis up to the 47th residue showed close homology between calf apo-AI and those of the mammals with which it was compared. However, residues 2, 7, 20 and 22 in calf AI (i.e. aspartic acid, serine, glutamic acid and isoleucine, respectively) were substituted by glutamic acid, proline or glutamine, aspartic acid, and valine or leucine respectively, in the other mammals.
S Auboiron; D A Sparrow; L Beaubatie; D Bauchart; J T Sparrow; P M Laplaud; M J Chapman
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  166     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-03-05     Completed Date:  1990-03-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  833-9     Citation Subset:  IM    
Laboratoire d'Etude du Métabolisme Energétique, INRA, Theix, Ceyrat, France.
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MeSH Terms
Age Factors
Amino Acid Sequence
Apolipoprotein A-I
Apolipoproteins A / analysis*
Cattle / physiology*
Lipoproteins, HDL / analysis*
Molecular Sequence Data
Grant Support
Reg. No./Substance:
0/Apolipoprotein A-I; 0/Apolipoproteins A; 0/Lipoproteins, HDL

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