Document Detail

Characterization of the amino acid response element within the human sodium-coupled neutral amino acid transporter 2 (SNAT2) System A transporter gene.
MedLine Citation:
PMID:  16445384     Owner:  NLM     Status:  MEDLINE    
The neutral amino acid transport activity, System A, is enhanced by amino acid limitation of mammalian cells. Of the three gene products that encode System A activity, the one that exhibits this regulation is SNAT2 (sodium-coupled neutral amino acid transporter 2). Fibroblasts that are deficient in the amino acid response pathway exhibited little or no induction of SNAT2 mRNA. Synthesis of SNAT2 mRNA increased within 1-2 h after amino acid removal from HepG2 human hepatoma cells. The amino acid responsive SNAT2 genomic element that mediates the regulation has been localized to the first intron. Increased binding of selected members of the ATF (activating transcription factor) and C/EBP (CCAAT/enhancer-binding protein) families to the intronic enhancer was established both in vitro and in vivo. In contrast, there was no significant association of these factors with the SNAT2 promoter. Expression of exogenous individual ATF and C/EBP proteins documented that specific family members are associated with either activation or repression of SNAT2 transcription. Chromatin immunoprecipitation analysis established in vivo that amino acid deprivation led to increased RNA polymerase II recruitment to the SNAT2 promoter.
Stela S Palii; Michelle M Thiaville; Yuan-Xiang Pan; Can Zhong; Michael S Kilberg
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  The Biochemical journal     Volume:  395     ISSN:  1470-8728     ISO Abbreviation:  Biochem. J.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-04-06     Completed Date:  2006-05-10     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  England    
Other Details:
Languages:  eng     Pagination:  517-27     Citation Subset:  IM    
Department of Biochemistry and Molecular Biology, Shands Cancer Center, and the Genetics Institute, University of Florida College of Medicine, Gainesville, FL 32610, USA.
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MeSH Terms
Activating Transcription Factors / genetics,  metabolism
Amino Acid Transport System A / genetics*,  metabolism*
Amino Acids / pharmacology*
Base Sequence
CCAAT-Enhancer-Binding Proteins / genetics,  metabolism
Cell Line
DNA / genetics,  metabolism
Eukaryotic Initiation Factor-2 / metabolism
Gene Expression Regulation / drug effects
Genome, Human / genetics
Molecular Sequence Data
Promoter Regions, Genetic / genetics
Protein Binding
Protein Kinases / metabolism
Protein-Serine-Threonine Kinases
RNA, Messenger / genetics
Response Elements / genetics*
Sodium / metabolism*
Transcription, Genetic / drug effects,  genetics
Grant Support
Reg. No./Substance:
0/Activating Transcription Factors; 0/Amino Acid Transport System A; 0/Amino Acids; 0/CCAAT-Enhancer-Binding Proteins; 0/Eukaryotic Initiation Factor-2; 0/RNA, Messenger; 0/SLC38A2 protein, human; 7440-23-5/Sodium; 9007-49-2/DNA; EC 2.7.-/Protein Kinases; EC protein, mouse; EC Kinases

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