Document Detail


Characterization of the aldose reductase-encoding gene family in rat.
MedLine Citation:
PMID:  1748296     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although the enzyme aldose reductase (AR) is implicated in the development of tissue pathology in diabetes, the exact mechanism of this involvement remains unclear. To better understand the role that expression of the aldose reductase-encoding gene (ALR) may play in diabetic complications, we have begun to analyze the gene and its regulatory regions, and we present here the sequence of four ALR genes in the rat. The putative functional gene is 14.1 kb long, has ten exons which show perfect sequence identity to the rat lens AR RNA sequence, and nine introns with classical splice-site consensus sequences. Potential regulatory elements in the 5'-flanking region of this gene include a TATA box and two CCAAT boxes. Probing rat genomic Southern blots with a fragment from the first intron indicates that there is probably only one copy of this gene in the rat genome. The other three genes are processed pseudogenes which show approx. 90% identity to the rat lens AR RNA sequence, contain no introns, and have poly(A) regions at their 3' ends. Chromosomal localization studies show the presence of ALR genes on chromosomes 3, 4 and 6 in the rat with the putative functional gene mapped on chromosome 4.
Authors:
C Graham; C Szpirer; G Levan; D Carper
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Gene     Volume:  107     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  1991 Nov 
Date Detail:
Created Date:  1992-01-21     Completed Date:  1992-01-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  259-67     Citation Subset:  IM    
Affiliation:
National Eye Institute, National Institutes of Health, Bethesda, MD 20892.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M60322;  S72785;  S72787;  S72789;  S72791;  S72793;  S72795;  S72797;  S72799;  S72801;  S72803
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MeSH Terms
Descriptor/Qualifier:
Aldehyde Reductase / genetics*
Animals
Base Sequence
Blotting, Southern
Chromosome Mapping
Consensus Sequence
Diabetes Mellitus, Experimental / genetics
Exons / genetics
Gene Expression / genetics
Hybrid Cells
Introns / genetics
Mice
Molecular Sequence Data
Multigene Family / genetics*
Pseudogenes / genetics*
Rats
Sequence Homology, Nucleic Acid
Chemical
Reg. No./Substance:
EC 1.1.1.21/Aldehyde Reductase

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