Document Detail


Characterization of the adhesive from cuvierian tubules of the sea cucumber Holothuria forskali (Echinodermata, Holothuroidea).
MedLine Citation:
PMID:  12925918     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sea cucumbers possess a peculiar specialized defense system: the so-called Cuvierian tubules. The system is mobilized when the animal is mechanically stimulated, resulting in the discharge of a few white filaments, the tubules. Their great adhesivity, combined with their high tensile strength, allows Cuvierian tubules to entangle and immobilize potential predators. The cellular origin and composition of the Cuvierian tubule adhesive were investigated in the species Holothuria forskali by studying prints left on the substratum after mechanical detachment of the tubule. Polyclonal antibodies raised against tubule print material were used to locate the origin of tubule print constituents in the tubules. Extensive immunoreactivity was detected in the secretory granules of mesothelial granular cells, suggesting that their secretions make up the bulk of the adhesive material. Tubule print material consists of 60% proteins and 40% carbohydrates, a composition that is unique among the adhesive secretions of marine invertebrates. Although it is highly insoluble, a small fraction of this material can be extracted using denaturing buffers. Electrophoretic analysis of the extracts revealed that it contains about 10 proteins with apparent molecular masses ranging from 17 to 220 kDa and with closely related amino acid compositions, rich in acidic and in small side-chain amino acids. The adhesive from the Cuvierian tubules of H. forskali shares these characteristics with many marine bioadhesives and structural biomaterials.
Authors:
Sonia DeMoor; J Herbert Waite; Michel Jangoux; Patrick Flammang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Marine biotechnology (New York, N.Y.)     Volume:  5     ISSN:  1436-2228     ISO Abbreviation:  Mar. Biotechnol.     Publication Date:    2003 Jan-Feb
Date Detail:
Created Date:  2003-08-19     Completed Date:  2003-11-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100892712     Medline TA:  Mar Biotechnol (NY)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  45-57     Citation Subset:  IM    
Affiliation:
Marine Biology Laboratory, University of Mons-Hainaut, 6 Avenue du Champ de Mars, B-7000 Mons, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Adhesives / chemistry*,  isolation & purification*
Animals
Behavior, Animal / physiology
Immunohistochemistry
Proteins / chemistry*,  isolation & purification*
Sea Cucumbers / chemistry*,  metabolism
Chemical
Reg. No./Substance:
0/Adhesives; 0/Proteins
Comments/Corrections
Erratum In:
Mar Biotechnol (NY). 2003 Jan-Feb;5(1):102

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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