Document Detail

Characterization of acute brain injuries and neurobehavioral profiles in a rabbit model of germinal matrix hemorrhage.
MedLine Citation:
PMID:  18845808     Owner:  NLM     Status:  MEDLINE    
BACKGROUND AND PURPOSE: Germinal matrix hemorrhage-intraventricular hemorrhage (GMH-IVH) is the most common neurological problem of premature infants and has enormous financial and social impact. Despite this, there is no standardized animal model of IVH depicting acute brain injuries. METHODS: We delivered rabbit-pups prematurely at 29-day gestation by C-section, administered intraperitoneal glycerol to the pups at 3-hour postnatal age to induce IVH, and evaluated the brain for evidence of injuries. RESULTS: About 80% of glycerol-treated pups developed gross IVH. We found greater neutrophil and microglia infiltration around the ventricles (periventricular zone) in pups with IVH than in controls. We noted more apoptosis and neuronal degeneration in the periventricular zone than in the neocortex in pups with IVH, but not in controls. There was evidence of axonal damage revealed by beta-amyloid precursor protein and neurofilament immunolabeling. Neurobehavioral testing showed that pups with IVH were more wobbly with lesser capability to walk on inclination than pups without IVH. There was no evidence of acute systemic toxicity in the glycerol-treated pups. An evaluation of autopsy materials from premature infants revealed similar evidence of apoptosis and cellular infiltration in the periventricular zone in cases with IVH, but not in cases without IVH-suggesting clinical relevance of the model. CONCLUSIONS: The study provides an instructive animal model of IVH with evidence of acute brain injuries that can be used to evaluate strategies in prevention of IVH and acute posthemorrhagic complications.
Paraskevi Georgiadis; Hongmin Xu; Caroline Chua; Furong Hu; Lee Collins; Chau Huynh; Edmund F Lagamma; Praveen Ballabh
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-10-09
Journal Detail:
Title:  Stroke; a journal of cerebral circulation     Volume:  39     ISSN:  1524-4628     ISO Abbreviation:  Stroke     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-25     Completed Date:  2009-01-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0235266     Medline TA:  Stroke     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3378-88     Citation Subset:  IM    
Department of Pediatrics, New York Medical College-Westchester Medical Center, Valhalla, NY, USA.
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MeSH Terms
Animals, Newborn
Behavior, Animal
Brain / pathology
Cerebral Hemorrhage / chemically induced*,  pathology,  psychology,  ultrasonography
Cerebral Ventricles / pathology*
Disease Models, Animal*
Gestational Age
Glycerol / administration & dosage,  toxicity
Infant, Newborn
Infant, Premature
Infant, Premature, Diseases / pathology
Injections, Intraperitoneal
Microglia / pathology
Nerve Degeneration / chemically induced,  etiology
Neutrophils / pathology
Premature Birth
Single-Blind Method
Grant Support
Reg. No./Substance:

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