| Characterization of the activities of p21Cip1/Waf1 promoter-driven reporter systems during camptothecin-induced senescence-like state of BHK-21 cells. | |
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MedLine Citation:
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PMID: 16779499 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It was attempted in this work to establish a cell line in which senescent cells can be readily and directly identified in situ in live culture. Transcriptional activation of p21(Cip1/Waf1) gene is known to be one of the key steps in the development of cellular senescence, whereas the elements within the p21(Cip1/Waf1) promoter that regulate the transcriptional activation of p21(Cip1/Waf1) during cellular senescence have not been clearly defined. Thus, several reporter plasmids were constructed in each of which the gene of green fluorescent protein was placed under the control of a selected fragment of p21(Cip1/Waf1) promoter, and stably transfected into BHK-21 cells. The transfected cells were induced to become senescence-like by camptothecin and assayed for fluorescence intensity. It was shown that the reporter system constructed with bases -2504 to +406 of the p21(Cip1/Waf1) promoter was very efficient in reflecting the senescence of BHK-21 cells by increased cytosolic fluorescence, and the fluorescence intensity of senescent cells was easily distinguished from that of quiescent cells. |
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Authors:
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Hsueh-Ling Cheng; Shiou-Ming Chang; Ya-Wen Cheng; Hung-Jen Liu; Yo-Chia Chen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-05-24 |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 291 ISSN: 0300-8177 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-10-12 Completed Date: 2006-12-19 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: Netherlands |
Other Details:
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Languages: eng Pagination: 29-38 Citation Subset: IM |
Affiliation:
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Institute of Biotechnology, National Pingtung University of Science and Technology, Taiwan, ROC. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects Camptothecin / pharmacology* Cell Aging / drug effects* Cell Cycle / drug effects Cricetinae Cyclin-Dependent Kinase Inhibitor p21 / genetics* Flow Cytometry Fluorescence Genes, Reporter* Green Fluorescent Proteins / metabolism Humans Plasmids / metabolism Promoter Regions, Genetic / genetics* Transfection beta-Galactosidase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/CDKN1A protein, human; 0/Cyclin-Dependent Kinase Inhibitor p21; 147336-22-9/Green Fluorescent Proteins; 7689-03-4/Camptothecin; EC 3.2.1.23/beta-Galactosidase |
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