Document Detail


Characterization of acid-induced molten globule like state of ficin.
MedLine Citation:
PMID:  19482042     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Effect of pH on the conformational behaviour of ficin (EC 3.4.22.3), a cysteine protease from the latex of Ficus carica was monitored by circular dichroism, fluorescence spectroscopy, ANS binding and hydrodynamic studies. The results obtained from near- and far-UV CD, intrinsic fluorescence and ANS binding studies demonstrate that ficin exhibits the characteristic properties of molten globule at acidic conditions between pH 1.4 and 2.0. Ficin at pH 1.4 retained about approximately 74% secondary structure with a substantial loss of tertiary structure. The acid-induced state was found to have a compact shape as measured by Stokes radius on size exclusion chromatography.
Authors:
K B Devaraj; Parigi Ramesh Kumar; V Prakash
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-29
Journal Detail:
Title:  International journal of biological macromolecules     Volume:  45     ISSN:  1879-0003     ISO Abbreviation:  Int. J. Biol. Macromol.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-07-31     Completed Date:  2009-10-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7909578     Medline TA:  Int J Biol Macromol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  248-54     Citation Subset:  IM    
Affiliation:
Department of Protein Chemistry and Technology, Central Food Technological Research Institute (A Constituent Laboratory of CSIR), Cheluvamba Mansion, KRS Road, Mysore 570020, India.
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MeSH Terms
Descriptor/Qualifier:
Acids / chemistry*
Acrylamide / chemistry
Chromatography, Gel
Circular Dichroism
Ficain / chemistry*
Ficus / chemistry
Guanidine / pharmacology
Hydrogen-Ion Concentration / drug effects
Protein Conformation
Protein Denaturation / drug effects
Spectrometry, Fluorescence
Temperature
Chemical
Reg. No./Substance:
0/Acids; 113-00-8/Guanidine; 79-06-1/Acrylamide; EC 3.4.22.3/Ficain

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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