Document Detail


Characterization of Wolbachia host cell range via the in vitro establishment of infections.
MedLine Citation:
PMID:  11823204     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Maternally transmitted bacteria of the genus Wolbachia are obligate, intracellular symbionts that are frequently found in insects and cause a diverse array of reproductive manipulations, including cytoplasmic incompatibility, male killing, parthenogenesis, and feminization. Despite the existence of a broad range of scientific interest, many aspects of Wolbachia research have been limited to laboratories with insect-rearing facilities. The inability to culture these bacteria outside of the invertebrate host has also led to the existing bias of Wolbachia research toward infections that occur in host insects that are easily reared. Here, we demonstrate that Wolbachia infections can be simply established, stably maintained, and cryogenically stored in vitro using standard tissue culture techniques. We have examined Wolbachia host range by introducing different Wolbachia types into a single tissue culture. The results show that an Aedes albopictus (Diptera: Culicidae) cell line can support five different Wolbachia infection types derived from Drosophila simulans (Diptera: Drosophilidae), Culex pipiens (Culicidae), and Cadra cautella (Lepidoptera: Phycitidae). These bacterial types include infection types that have been assigned to two of the major Wolbachia clades. As an additional examination of Wolbachia host cell range, we demonstrated that a Wolbachia strain from D. simulans could be established in host insect cell lines derived from A. albopictus, Spodoptera frugiperda (Lepidoptera: Noctuidae), and Drosophila melanogaster. These results will facilitate the development of a Wolbachia stock center, permitting novel approaches for the study of Wolbachia infections and encouraging Wolbachia research in additional laboratories.
Authors:
Stephen L Dobson; Eric J Marsland; Zoe Veneti; Kostas Bourtzis; Scott L O'Neill
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  68     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-02-01     Completed Date:  2002-03-28     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  656-60     Citation Subset:  IM    
Affiliation:
Section of Vector Biology, Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, CT 06437, USA. sdobson@uky.edu
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MeSH Terms
Descriptor/Qualifier:
Aedes / microbiology*
Animals
Cell Culture Techniques / methods
Cells, Cultured
Culex / microbiology
Drosophila / microbiology
Drosophila melanogaster / microbiology*
Lepidoptera / microbiology
Spodoptera / microbiology*
Wolbachia / growth & development*,  isolation & purification,  pathogenicity*
Grant Support
ID/Acronym/Agency:
AI 40620/AI/NIAID NIH HHS
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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