Document Detail

Characterization of WdChs3p, a class III chitin synthase, of Wangiella (Exophiala) dermatitidis, overexpressed in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  12146758     Owner:  NLM     Status:  MEDLINE    
The class III chitin synthase (WdChs3p) of the fungal pathogen of humans Wangiella dermatitidis is expressed differentially under stress growth conditions and, together with WdChs2p, it contributes to virulence. However, because this fungus produces four other chitin synthases, and a quadruple disruption strain with only WdChs3p activity has not been derived, we characterized this enzyme further after heterologous expression of the WdCHS3 cDNA under the regulation of the inducible GAL1 promoter in Saccharomyces cerevisiae. In contrast to our prior conclusions about the zymogenic nature of WdChs3p produced in W. dermatitidis, the enzyme expressed in yeast had a non-zymogenic activity, that is, an activity not potentiated by controlled proteolysis. Nonetheless, other characteristics of WdChs3p activity expressed in yeast were similar to those of the enzyme from its parent, including being stable over a broad range of pH and temperature values, which should facilitate attempts to purify this unique chitin synthase and better define its structure.
Z Wang; P J Szaniszlo
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Medical mycology     Volume:  40     ISSN:  1369-3786     ISO Abbreviation:  Med. Mycol.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-07-30     Completed Date:  2003-05-07     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  9815835     Medline TA:  Med Mycol     Country:  England    
Other Details:
Languages:  eng     Pagination:  283-9     Citation Subset:  IM    
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MeSH Terms
Chitin / metabolism
Chitin Synthase / biosynthesis,  genetics*,  metabolism
DNA, Complementary / biosynthesis
Exophiala / enzymology,  genetics*
Fungal Proteins / biosynthesis,  genetics*,  metabolism
Genetic Vectors
Hydrogen-Ion Concentration
Recombinant Proteins / metabolism
Saccharomyces cerevisiae / metabolism
Grant Support
Reg. No./Substance:
0/DNA, Complementary; 0/Fungal Proteins; 0/Recombinant Proteins; 1398-61-4/Chitin; EC Synthase

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