Document Detail


Characterization of Theileria species by PCR using specific target sequences.
MedLine Citation:
PMID:  11071548     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Theileriosis is an infectious disease in tropical countries and in the Mediterranean area. It is caused by Theileria, a haemoprotozoan, transmitted by vectors belonging to the Ixodidae. In Southern Italy and in Sicily the infection is due mainly to T. annulata, but in some cases other species are involved in the disease. The authors describe a method to identify theileriosis in cattle blood samples, using PCR and hybridization techniques. Different primer sets were used to amplify different DNA target sequences, both genus and species specific. Blood samples from cattle were collected in Sicily. The DNA extracted from blood samples was employed first to detect the presence of the 18S ribosomal subunit gene specific for Theileria genus. Successively the positive samples were analysed to identify the species, T. annulata or T. buffeli/orientalis, using as target sequences for amplification respectively a fragment of the TAMS-1 and p33/34 antigens gene. Here the authors describe for the first time the presence of T. buffeli/orientalis infection in Sicilian herds. In fact 66% of positive blood samples were T. buffeli/orientalis infected.
Authors:
L Maxia; S Reale; G R Loria; A Greco; F Vitale; N S Glorioso; O Sparagano
Related Documents :
15131148 - Comparison of a duplex quantitative real-time pcr assay and the cobas amplicor cmv moni...
23831448 - Deep sequencing as a method of typing bluetongue virus isolates.
20002568 - Verification of an edwardsiella ictaluri-specific diagnostic pcr.
24833218 - Genetic and evolutionary characterization of venezuelan equine encephalitis virus isola...
11549318 - Identification and characterization of three members of a novel subclass of protocadher...
9971778 - Mechanisms that regulate epstein-barr virus ebna-1 gene transcription during restricted...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Parassitologia     Volume:  41 Suppl 1     ISSN:  0048-2951     ISO Abbreviation:  Parassitologia     Publication Date:  1999 Sep 
Date Detail:
Created Date:  2000-12-19     Completed Date:  2000-12-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0413724     Medline TA:  Parassitologia     Country:  ITALY    
Other Details:
Languages:  eng     Pagination:  69-72     Citation Subset:  IM    
Affiliation:
Istituto Zooprofilattico Sperimentale della Sicilia A. Mirri, Palermo, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Southern / veterinary
Cattle
Cattle Diseases / diagnosis,  epidemiology*,  parasitology
Electrophoresis, Agar Gel / veterinary
Polymerase Chain Reaction / veterinary*
Sicily / epidemiology
Theileria / classification*,  genetics
Theileriasis / diagnosis,  epidemiology*,  parasitology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Clinical and laboratory studies on theileriosis outbreaks in Sicily, Italy.
Next Document:  Development of sero-diagnostic and molecular tools for the control of important tick-borne pathogens...