Document Detail

Characterization of Theileria species by PCR using specific target sequences.
MedLine Citation:
PMID:  11071548     Owner:  NLM     Status:  MEDLINE    
Theileriosis is an infectious disease in tropical countries and in the Mediterranean area. It is caused by Theileria, a haemoprotozoan, transmitted by vectors belonging to the Ixodidae. In Southern Italy and in Sicily the infection is due mainly to T. annulata, but in some cases other species are involved in the disease. The authors describe a method to identify theileriosis in cattle blood samples, using PCR and hybridization techniques. Different primer sets were used to amplify different DNA target sequences, both genus and species specific. Blood samples from cattle were collected in Sicily. The DNA extracted from blood samples was employed first to detect the presence of the 18S ribosomal subunit gene specific for Theileria genus. Successively the positive samples were analysed to identify the species, T. annulata or T. buffeli/orientalis, using as target sequences for amplification respectively a fragment of the TAMS-1 and p33/34 antigens gene. Here the authors describe for the first time the presence of T. buffeli/orientalis infection in Sicilian herds. In fact 66% of positive blood samples were T. buffeli/orientalis infected.
L Maxia; S Reale; G R Loria; A Greco; F Vitale; N S Glorioso; O Sparagano
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Parassitologia     Volume:  41 Suppl 1     ISSN:  0048-2951     ISO Abbreviation:  Parassitologia     Publication Date:  1999 Sep 
Date Detail:
Created Date:  2000-12-19     Completed Date:  2000-12-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0413724     Medline TA:  Parassitologia     Country:  ITALY    
Other Details:
Languages:  eng     Pagination:  69-72     Citation Subset:  IM    
Istituto Zooprofilattico Sperimentale della Sicilia A. Mirri, Palermo, Italy.
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MeSH Terms
Blotting, Southern / veterinary
Cattle Diseases / diagnosis,  epidemiology*,  parasitology
Electrophoresis, Agar Gel / veterinary
Polymerase Chain Reaction / veterinary*
Sicily / epidemiology
Theileria / classification*,  genetics
Theileriasis / diagnosis,  epidemiology*,  parasitology

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