| Characterization of a Staphylococcus aureus surface virulence factor that promotes resistance to oxidative killing and infectious endocarditis. | |
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MedLine Citation:
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PMID: 20937760 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Staphylococcus aureus is a prominent human pathogen and a leading cause of community- and hospital-acquired bacterial infections worldwide. Herein, we describe the identification and characterization of the S. aureus 67.6-kDa hypothetical protein, named for the surface factor promoting resistance to oxidative killing (SOK) in this study. Sequence analysis showed that the SOK gene is conserved in all sequenced S. aureus strains and homologous to the myosin cross-reactive antigen of Streptococcus pyogenes. Immunoblotting and immunofluorescence analysis showed that SOK was copurified with membrane fractions and was exposed on the surface of S. aureus Newman and RN4220. Comparative analysis of wild-type S. aureus and an isogenic deletion strain indicated that SOK contributes to both resistance to killing by human neutrophils and to oxidative stress. In addition, the S. aureus sok deletion strain showed dramatically reduced aortic valve vegetation and bacterial cell number in a rabbit endocarditis model. These results, plus the suspected role of the streptococcal homologue in certain diseases such as acute rheumatic fever, suggest that SOK plays an important role in cardiovascular and other staphylococcal infections. |
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Authors:
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Natalia Malachowa; Petra L Kohler; Patrick M Schlievert; Olivia N Chuang; Gary M Dunny; Scott D Kobayashi; Jacek Miedzobrodzki; Gregory A Bohach; Keun Seok Seo |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-10-11 |
Journal Detail:
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Title: Infection and immunity Volume: 79 ISSN: 1098-5522 ISO Abbreviation: Infect. Immun. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-23 Completed Date: 2011-01-25 Revised Date: 2013-04-01 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: United States |
Other Details:
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Languages: eng Pagination: 342-52 Citation Subset: IM |
Affiliation:
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Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacterial Proteins / genetics, metabolism* Computational Biology Endocarditis, Bacterial / microbiology* Gene Deletion Gene Expression Regulation, Bacterial / physiology Humans Neutrophils / physiology Oxidative Stress Polymorphism, Restriction Fragment Length Rabbits Staphylococcus aureus / metabolism* Virulence Factors / chemistry, genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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AI074283/AI/NIAID NIH HHS; P20 RR016454/RR/NCRR NIH HHS; P20-RR016454/RR/NCRR NIH HHS; P20-RR15587/RR/NCRR NIH HHS; P20RR015587/RR/NCRR NIH HHS; R01 AI074283-23/AI/NIAID NIH HHS; U54 AI57153/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Virulence Factors |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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