Document Detail

Characterization of a Schwann cell enhancer in the myelin basic protein gene.
MedLine Citation:
PMID:  15525335     Owner:  NLM     Status:  MEDLINE    
Oligodendrocytes and Schwann cells use diverse regulatory elements to transcribe myelin basic protein (Mbp). For example, an enhancer 9.0 kb upstream of Mbp (MbpSCE1) activated either the proximal Mbp or hsp68 promoters only in Schwann cells in transgenic mice. Here, we analyze MbpSCE1 in vitro and in vivo and show that MbpSCE1 also activates another myelin gene, Mpz, specifically in Schwann cells in transgenic mice. Surprisingly, although MbpSCE1 behaves as an enhancer in Schwann cells, we show here for the first time that it also activates transcription robustly in cultured oligodendrocytes, but only from its original genomic position. Diverse nuclear proteins binding at distinct combinations of DNA elements in the two cell types may account for cell-specific MbpSCE1 function. A further surprise is that MbpSCE1 activation does not depend on consensus binding sites for the myelin-associated PPARbeta or Krox 20 transcription factors. Finally, chromatin context augments activation of MbpSCE1. Therefore, MbpSCE1 is active in both Schwann cells and oligodendrocytes, but by diverse mechanisms.
Carla Taveggia; Antonella Pizzagalli; Ernesta Fagiani; Albee Messing; M Laura Feltri; Lawrence Wrabetz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  91     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  2004 Nov 
Date Detail:
Created Date:  2004-11-04     Completed Date:  2005-05-25     Revised Date:  2012-02-22    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  813-24     Citation Subset:  IM    
San Raffaele Scientific Institute, DIBIT, Milan, Italy.
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MeSH Terms
Cells, Cultured
Chromatin / metabolism
DNA Footprinting
Deoxyribonuclease I
Electrophoretic Mobility Shift Assay
Enhancer Elements, Genetic / genetics,  physiology*
Genes, Reporter
Mice, Transgenic
Myelin Basic Proteins / genetics*
Nuclear Proteins / metabolism
Oligodendroglia / cytology,  metabolism
Rats, Sprague-Dawley
Schwann Cells / cytology,  metabolism*
Sequence Analysis, DNA
Sequence Deletion
Grant Support
Reg. No./Substance:
0/Chromatin; 0/Myelin Basic Proteins; 0/Nuclear Proteins; EC I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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