Document Detail


Characterization of Salmonella serovars by PCR-single-strand conformation polymorphism analysis.
MedLine Citation:
PMID:  12089246     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PCR-restriction fragment length polymorphism (PCR-RFLP) and PCR-single-strand conformation polymorphism (PCR-SSCP) analyses were carried out on the 1.6-kb groEL gene from 41 strains of 10 different Salmonella serovars. Three HaeIII RFLP profiles were recognized, but no discrimination between the serovars could be achieved by this technique. However, PCR-SSCP analysis of the groEL genes of various Salmonella serovars produced 14 SSCP profiles, indicating the potential of this technique to differentiate different Salmonella serovars (interserovar differentiation). Moreover, PCR-SSCP could differentiate strains within a subset of serovars (intraserovar discrimination), as three SSCP profiles were produced for the 11 Salmonella enterica serovar Enteritidis strains, and two SSCP profiles were generated for the 7 S. enterica serovar Infantis and five S. enterica serovar Newport strains. PCR-SSCP has the potential to complement classical typing methods such as serotyping and phage typing for the typing of Salmonella serovars due to its rapidity, simplicity, and typeability.
Authors:
Satheesh Nair; Thong Kwai Lin; Tikki Pang; Martin Altwegg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  40     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2002 Jul 
Date Detail:
Created Date:  2002-06-28     Completed Date:  2002-08-26     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2346-51     Citation Subset:  IM    
Affiliation:
Institute of Postgraduate Studies & Research, University of Malaya, Kuala Lumpur, Malaysia. h0sathe@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Bacterial Typing Techniques
Chaperonin 60 / genetics
DNA, Bacterial / genetics,  isolation & purification
Genes, Bacterial
Humans
Mutation
Polymerase Chain Reaction / methods*
Polymorphism, Restriction Fragment Length
Polymorphism, Single-Stranded Conformational*
Salmonella / classification*,  genetics*,  isolation & purification
Salmonella Infections / diagnosis,  microbiology
Serotyping
Species Specificity
Chemical
Reg. No./Substance:
0/Chaperonin 60; 0/DNA, Bacterial
Comments/Corrections

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