Document Detail


Characterization of Rift Valley fever virus transcriptional terminations.
MedLine Citation:
PMID:  17537856     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rift Valley fever virus (RVFV) (genus Phlebovirus, family Bunyaviridae) has a tripartite negative-strand genome and causes a mosquito-borne disease among humans and livestock in sub-Saharan African and Arabian Peninsula countries. Phlebovirus L, M, and N mRNAs are synthesized from the virus-sense RNA segments, while NSs mRNA is transcribed from the anti-virus-sense S segment. The present study determined the 3' termini of all RVFV mRNAs. The 3' termini of N and NSs mRNAs were mapped within the S-segment intergenic region and were complementary to each other by 30 to 60 nucleotides. The termini of M and L mRNAs were mapped within 122 to 107 nucleotides and 16 to 41 nucleotides, respectively, from the 5' ends of their templates. Viral RNA elements that control phlebovirus transcriptional terminations are largely unknown. Our studies suggested the importance of a pentanucleotide sequence, CGUCG, for N, NSs, and M mRNA transcription terminations. Homopolymeric tracts of C sequences, which were located upstream of the pentanucleotide sequence, promoted N and M mRNA terminations. Likewise, the homopolymeric tracts of G sequences that are found upstream of the pentanucleotide sequence promoted NSs mRNA termination. The L-segment 5'-untranslated region (L-5' UTR) had neither the pentanucleotide sequence nor homopolymeric sequences, yet replacement of the S-segment intergenic region with the L-5' UTR exerted N mRNA termination in an infectious virus. The L-5' UTR contained two 13-nucleotide-long complete complementary sequences, and their sequence complementarities were important for L mRNA termination. A computer-mediated RNA secondary structure analysis further suggested that RNA secondary structures formed by the sections of the two 13-nucleotide-long sequences and by the sequence between them may have a role in L mRNA termination. Our data demonstrated that viral RNA elements that govern L mRNA termination differed from those that regulate mRNA terminations in the M and S segments.
Authors:
Tetsuro Ikegami; Sungyong Won; C J Peters; Shinji Makino
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-05-30
Journal Detail:
Title:  Journal of virology     Volume:  81     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-07-26     Completed Date:  2007-09-13     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8421-38     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555-1019, USA. shmakino@utmb.edu
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MeSH Terms
Descriptor/Qualifier:
3' Untranslated Regions / chemistry*,  metabolism
Base Sequence
Gene Expression Regulation, Viral*
Genome, Viral / genetics
Molecular Sequence Data
Nucleic Acid Conformation
RNA, Viral / chemistry*,  metabolism
Rift Valley fever virus / genetics*
Sequence Analysis, RNA
Terminator Regions, Genetic*
Transcription, Genetic
Grant Support
ID/Acronym/Agency:
U54 AI 057156/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/3' Untranslated Regions; 0/RNA, Viral
Comments/Corrections

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