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Characterization of Polyhydroxyalkanotes accumulated by a Moderately Halophilic Salt pan isolate Bacillus megaterium strain H16.
MedLine Citation:
PMID:  23311612     Owner:  NLM     Status:  Publisher    
AIMS: Characterization of polyhydroxyalkanotes (PHA) accumulated by halophilic bacteria isolated from solar salterns. METHODS AND RESULTS: Twenty six halophilic isolates were obtained from solar salterns of Goa, India. They were screened for accumulation of PHA by Sudan black B, Nile blue A and Nile red stain. Strains H15, H16 and H26 were selected based on their intensity of Nile blue A/Nile red fluorescence. On the basis of phenotypic and genotypic characterization the three isolates were identified as Bacillus megaterium. Growth kinetics and polymer accumulating capacity of strain H16 was studied in E2 mineral media with 2% glucose with/without NaCl. In the absence of NaCl strain H16 accumulated PHA to 40.0% (w/w) of CDW at 42 hours of growth. Whereas in presence of 5% w/v NaCl the culture showed longer lag phase of up to 24 hours and accumulated a maximum PHA of 39% (w/w) CDW at 54(th) hour of growth. The Infra red spectra of both the polymers exhibited peaks at 1733.9 cm(-1) characteristic of C=O. Scans of (1) H NMR showed a doublet at 2.5 ppm corresponding to methylene group (-CH(2) ), the signal at 5.3 ppm corresponded to methine group (-CH-), and another signal at 1.3 ppm corresponded to the methyl group (-CH(3) ). Scans of (13) C NMR showed prominent peaks at 20 ppm, 40 ppm, 67-68 ppm and 170 ppm indicating the polymer to be homopolymer of 3-hydroxybutyrates. The polymer is stable up to a temperature of 160°C. CONCLUSIONS: Three moderately halophilic isolates (strain H15, H16 and H26) capable of accumulating PHA were isolated from solar salterns of Ribandar Goa, India and identified as Bacillus megaterium based on phenotypic and genotypic characterization. Strain H16 accumulated Polyhydroxybutyrate in presence and absence of NaCl up to 40% of its CDW. © 2013 The Authors Journal of Applied Microbiology © 2013 The Society for Applied Microbiology.
B B Salgaonkar; Kabilan Mani; J M Braganca
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-13
Journal Detail:
Title:  Journal of applied microbiology     Volume:  -     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 The Authors Journal of Applied Microbiology © 2013 The Society for Applied Microbiology.
Department of Biological Sciences, Birla Institute of Technology and Science Pilani, K K Birla, Goa Campus, Zuarinagar, Goa, India, 403 726.
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