Document Detail


Characterization of PSII-LHCII supercomplexes isolated from pea thylakoid membrane by one-step treatment with α- and β-dodecyl-D-maltoside.
MedLine Citation:
PMID:  23148265     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It was the work of Jan Anderson, together with Keith Boardman, that showed it was possible to physically separate photosystem I (PSI) from photosystem II (PSII), and it was Jan Anderson who realized the importance of this work in terms of the fluid-mosaic model as applied to the thylakoid membrane. Since then, there has been a steady progress in the development of biochemical procedures to isolate PSII and PSI both for physical and structural studies. Dodecylmaltoside (DM) has emerged as an effective mild detergent for this purpose. DM is a glucoside-based surfactant with a bulky hydrophilic head group composed of two sugar rings and a non-charged alkyl glycoside chain. Two isomers of this molecule exist, differing only in the configuration of the alkyl chain around the anomeric centre of the carbohydrate head group, axial in α-DM and equatorial in β-DM. We have compared the use of α-DM and β-DM for the isolation of supramolecular complexes of PSII by a single-step solubilization of stacked thylakoid membranes isolated from peas. As a result, we have optimized conditions to obtain homogeneous preparations of the C(2)S(2)M(2) and C(2)S(2) supercomplexes following the nomenclature of Dekker & Boekema (2005 Biochim. Biophys. Acta 1706, 12-39). These PSII-LHCII supercomplexes were subjected to biochemical and structural analyses.
Authors:
Simone Barera; Cristina Pagliano; Tillmann Pape; Guido Saracco; James Barber
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Philosophical transactions of the Royal Society of London. Series B, Biological sciences     Volume:  367     ISSN:  1471-2970     ISO Abbreviation:  Philos. Trans. R. Soc. Lond., B, Biol. Sci.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-13     Completed Date:  2013-04-22     Revised Date:  2014-01-10    
Medline Journal Info:
Nlm Unique ID:  7503623     Medline TA:  Philos Trans R Soc Lond B Biol Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  3389-99     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Calcium Chloride / chemistry
Crystallography, X-Ray
Cyanobacteria / chemistry
Electrophoresis, Polyacrylamide Gel
Glucosides / chemistry*
Light-Harvesting Protein Complexes / chemistry*,  isolation & purification
Models, Molecular
Oxygen / chemistry
Peas / chemistry*
Photosystem II Protein Complex / chemistry*,  isolation & purification
Protein Binding
Thylakoid Membrane Proteins / chemistry,  isolation & purification*
Thylakoids / chemistry*
Ultracentrifugation / methods
Chemical
Reg. No./Substance:
0/Glucosides; 0/Light-Harvesting Protein Complexes; 0/Photosystem II Protein Complex; 0/Thylakoid Membrane Proteins; 69227-93-6/dodecyl maltoside; M4I0D6VV5M/Calcium Chloride; S88TT14065/Oxygen
Comments/Corrections

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