Document Detail

Characterization of Novel EGs Reconstructed from Bacillus subtilis Endoglucanase.
MedLine Citation:
PMID:  23340868     Owner:  NLM     Status:  Publisher    
Bacterial cellulases have taken on satisfactory application performance and economic value in detergent industry. Neutral endoglucanase (EG1) gene was cloned from Bacillus subtilis and expressed Pichia pastoris in our previous study. Redesigned endoglucanases enhanced cellulase domain, added and deleted carbohydrate-binding module (CBM), named EG2, EG3, and EG4, respectively, were constructed in this study. The redesigned EG genes were expressed in P. pastoris, and their characters were also discussed. The optimal temperature and pH value of the all EGs was 65 °C and 6.0, respectively, where their enzymatic activities in P. pastoris cultivation supernatant reached 867, 651, 966, and 881 U/mL. EG2 showed 24.9 % enzymatic activity loss compared to natural endoglucanase. EG4 showed specific activity 30 % loss and thermostability decrease compared to EG1, which suggested CBM played an important role in improving the catalytic power and heat stability of cellulase family which attached. The specific activity of EG2 and EG3 showed similar to EG1, which suggested neither enhancement of CD nor CBM to endoglucanase can improve its catalytic power, which might rest with its intact topologic structure.
Tang Zi-Zhong; Wu Zhen-Fang; Chen Hui; Lai Xin; Han Xue-Yi; Wu Qi
Related Documents :
25287348 - Studying bovine early embryo transcriptome by microarray.
23998118 - Identification of pentatricopeptide repeat proteins in the model organism dictyostelium...
24240858 - Nuclear-mitochondrial interactions in cytoplasmic male-sterile sorghum.
25120168 - Biochemical and molecular characterization of avena indolines and their role in kernel ...
19436228 - Elevation of somatic ca2+ upregulates genes nr4a1 and egr2, but not bdnf and arc.
11395438 - Amada: analysis of microarray data.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-23
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  -     ISSN:  1559-0291     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
College of Life and Science, Sichuan Agricultural University, Ya'an, 625014, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Structural Insights on Mycobacterium tuberculosis Thiazole Synthase-A Molecular Dynamics/Docking App...
Next Document:  Plant Regeneration Through Callus Organogenesis and True-to-Type Conformity of Plants by RAPD Analys...