Document Detail


Characterization of NorR protein, a multifunctional regulator of norA expression in Staphylococcus aureus.
MedLine Citation:
PMID:  12730173     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We characterized a Staphylococcus aureus norA gene expression regulator, NorR, initially identified from its binding to the norA promoter. The norR gene was 444 bp in length, located approximately 7 kb upstream from the norA gene, and encoded a predicted 17.6-kDa protein. Overexpression of norR in wild-type S. aureus strain ISP794 led to a fourfold decrease in sensitivity to quinolones and ethidium bromide and an increase in the level of norA transcripts, suggesting that NorR acts as a positive regulator of norA expression. Overexpression of norR in sarA and agr mutants did not alter quinolone sensitivity or levels of norA transcription, indicating that the presence of these two global regulatory systems is necessary for NorR to affect the expression of norA. Insertion and disruption of norR in ISP794 increased resistance to quinolones by 4- to 16-fold but had no effect on norA transcription, suggesting that NorR acts as a repressor for another unidentified efflux pump or pumps. These mutants also exhibited an exaggerated clumping phenotype in liquid media, which was complemented fully by a plasmid-encoded norR gene. Collectively, these results indicate that NorR is a multifunctional regulator, affecting cell surface properties as well as the expression of NorA and likely other multidrug resistance efflux pumps.
Authors:
Que Chi Truong-Bolduc; Xiamei Zhang; David C Hooper
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  185     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-05-05     Completed Date:  2003-06-16     Revised Date:  2013-07-23    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3127-38     Citation Subset:  IM    
Affiliation:
Division of Infectious Diseases and Medical Services, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114-2696, USA.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics*,  isolation & purification,  metabolism*
Base Sequence
Binding Sites
Cell Membrane / genetics
Drug Resistance, Multiple, Bacterial / genetics
Gene Expression Regulation, Bacterial*
Genes, Regulator
Molecular Sequence Data
Multidrug Resistance-Associated Proteins
Mutation
Promoter Regions, Genetic
Quinolones / pharmacology
RNA, Messenger / metabolism
Staphylococcus aureus / drug effects,  genetics*,  metabolism
Transcription Factors / genetics*,  isolation & purification,  metabolism*
Transcription, Genetic
Grant Support
ID/Acronym/Agency:
R01 AI023988/AI/NIAID NIH HHS; R01 AI23988/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Multidrug Resistance-Associated Proteins; 0/NorR protein, Staphylococcus aureus; 0/Quinolones; 0/RNA, Messenger; 0/Transcription Factors; 133135-40-7/NorA protein, Staphylococcus
Comments/Corrections

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