|Characterization of Mycobacterium orygis.|
|Jump to Full Text|
|PMID: 23017199 Owner: NLM Status: MEDLINE|
|Nicolaas C Gey van Pittius; Paul D van Helden; Robin M Warren|
Related Documents :
|23461889 - Examining the nature of the association between attention-deficit hyperactivity disorde...
23589099 - Profiling disordered eating patterns and body mass index (bmi) in the english general p...
12929979 - Molecular diagnosis of dysmorphic syndromes and inherited metabolic disorders in thailand.
9850739 - The feasibility of replacement therapy for inherited disorder of glycolysis: triosephos...
19030119 - Diagnostic approaches to pediatric cardiomyopathy of metabolic genetic etiologies and t...
23552429 - Do race, ethnicity, and psychiatric diagnoses matter in the prevalence of multiple chro...
12121209 - Test-retest reliability of self-reported age at onset of selected psychiatric diagnoses...
10758249 - Serotonin receptor 2a, 2c, 1a genes and response to lithium prophylaxis in mood disorders.
12670609 - An empirically derived taxonomy of common distress syndromes in the medically ill.
|Type: Comment; Letter|
|Title: Emerging infectious diseases Volume: 18 ISSN: 1080-6059 ISO Abbreviation: Emerging Infect. Dis. Publication Date: 2012 Oct|
|Created Date: 2012-09-28 Completed Date: 2013-02-19 Revised Date: 2013-04-08|
Medline Journal Info:
|Nlm Unique ID: 9508155 Medline TA: Emerg Infect Dis Country: United States|
|Languages: eng Pagination: 1708-9 Citation Subset: IM|
|APA/MLA Format Download EndNote Download BibTex|
Mycobacterium tuberculosis / classification*
Tuberculosis / microbiology*
|Emerg Infect Dis. 2012 Apr;18(4):653-5
Journal ID (nlm-ta): Emerg Infect Dis
Journal ID (iso-abbrev): Emerging Infect. Dis
Journal ID (publisher-id): EID
Publisher: Centers for Disease Control and Prevention
Print publication date: Month: 10 Year: 2012
Volume: 18 Issue: 10
First Page: 1708 Last Page: 1709
PubMed Id: 23017199
Publisher Id: 12-0569
|Characterization of Mycobacterium orygis Alternate Title:Characterization of Mycobacterium orygis|
|Nicolaas C. Gey van Pittius|
|Paul D. van Helden|
|Robin M. Warren|
|Stellenbosch University Faculty of Medicine and Health Sciences, Tygerberg, South Africa
|Correspondence: Address for correspondence: Nicolaas C. Gey van Pittius, DST/NRF Centre of Excellence for Biomedical TB Research/MRC Centre for Molecular and Cellular Biology/Division of Molecular Biology and Human Genetics, Stellenbosch University Faculty of Medicine and Health Sciences, PO Box 19063, Tygerberg 7505, South Africa; email: email@example.com
To the Editor: In a recently published study, van Ingen et al. (1) described the molecular characterization and phylogenetic position of the oryx bacillus, a member of the Mycobacterium tuberculosis complex, and proposed a long overdue name for the organism: Mycobacterium orygis. The authors described oryx bacillus as a separate taxon; the aim was for this description to be used in the future to identify the subspecies. Thus, we thought it pertinent to provide additional information that would be useful in speciating isolates of the oryx bacillus.
In a recent study, we genotyped an isolate of oryx bacillus obtained from an African buffalo in South Africa (2). This isolate was typed by using 16S rDNA, M. tuberculosis complex–specific multiplex-PCR, regions-of-difference analyses, gyrase B gene single nucleotide polymorphism (SNP) analysis, spoligotyping, and mycobacterial interspersed repetitive units–variable number tandem repeat typing. We showed that, in addition to the markers described by van Ingen et al. (1), regions of difference 701 and 702 were also intact in M. orygis.
In addition, van Ingen et al. identified the Rv204238 GGC mutation as a novel, useful genetic marker to identify M. orygis. However, such a marker already exists in the form of the very specific gyrBoryx G to A SNP at position 1113, which was described by Huard et al. (3). On its own, SNP detection in the gyrB gene allows differentiation of at least 6 of the 9 M. tuberculosis complex species from each other (M. canettii, M. tuberculosis, M. orygis, M. microti, M. caprae, and M. bovis) (3). Thus, the SNP at position 1113 is more useful than the Rv204238 mutation as a novel and distinct genetic marker to identify M. orygis.
Apart from this, we found that the sequence type (ST) 587 was not the only spoligotype specific for M. orygis. In our study, the variant type ST701 (annotated as M. africanum in the spolDB4 database) (4) is also an M. orygis–specific type and exactly matches that of a previous isolate of the oryx bacillus (SB0319) from the M. bovis spoligotype database (5). This spoligotype differs from ST587 by the presence of spacer 18, and the spoligotype was not found in the extensive sample set of van Ingen et al. (1).
Suggested citation for this article: Gey van Pittius NC, van Helden PD, Warren RM. Characterization of Mycobacterium orygis [letter]. Emerg Infect Dis [Internet]. 2012 Oct [date cited]. http://dx.doi.org/10.3201/eid1810.120569
|1. .||van IngenJ, RahimZ, MulderA, BoereeMJ, SimeoneR, BroschR, et al. Characterization of Mycobacterium orygis as M. tuberculosis complex subspecies.Emerg Infect Dis. Year: 2012;18:653–510.3201/eid1804.11088822469053|
|2. .||Gey van PittiusNC, PerrettKD, MichelAL, KeetDF, HlokweT, StreicherEM, et al. Infection of African buffalo (Syncerus caffer) by oryx bacillus, a rare member of the antelope clade of the Mycobacterium tuberculosis complex.J Wildl Dis. In press.|
|3. .||HuardRC, FabreM, de HaasP, Oliveira LazzariniLC, van SoolingenD, CousinsD, et al. Novel genetic polymorphisms that further delineate the phylogeny of the Mycobacterium tuberculosis complex.J Bacteriol. Year: 2006;188:4271–8710.1128/JB.01783-0516740934|
|4. .||BrudeyK, DriscollJR, RigoutsL, ProdingerWM, GoriA, Al-HajojSA, et al. Mycobacterium tuberculosis complex genetic diversity: mining the fourth international spoligotyping database (SpolDB4) for classification, population genetics and epidemiology.BMC Microbiol. Year: 2006;6:2310.1186/1471-2180-6-2316519816|
|5. .||Mbovis.orgM. bovis spoligotype database. Year: 2008 [cited 2012 Jun 29]. http://www.mbovis.org/spoligodatabase/intro.htm|
Keywords: Keywords: Mycobacterium tuberculosis complex, Mycobacterium orygis, oryx bacillus, genotyping, characterization, bacteria, tuberculosis and other mycobacteria.
Previous Document: The influence of grip on oxygen consumption and leg forces when using classical style roller skis.
Next Document: Quetiapine monotherapy in acute phase for major depressive disorder: a meta-analysis of randomized, ...