Document Detail


Characterization of Lgr5-positive epithelial cells in the murine thymus.
MedLine Citation:
PMID:  23423968     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Leucine-rich repeat-containing G protein-coupled receptor (Lgr)5 is a marker for epithelial stem cells in the adult intestine of mice. Lgr5 transcripts have also been detected in the developing murine thymus, leading to speculation that Lgr5 is a marker for the long-sought stem cell of the thymus. To address the nature of the Lgr5-expressing thymic epithelial cells (TECs), we used Lgr5-GFP reporter mice. We show that epithelial cells expressing Lgr5 protein are present in the fetal thymus during a specific developmental window yet are no longer detectable at birth. To analyze the function of the Lgr5 protein during thymus development, we generated Lgr5(-/-) mice. These experiments unequivocally show that thymus development is not perturbed in the absence of Lgr5, that all TEC subsets develop in Lgr5(-/-) mice and that T cells are produced in the expected ratios. Finally, by using an inducible lineage tracing system to track the progeny of Lgr5(+) fetal TECs in vivo, we demonstrated that Lgr5(+) fetal TECs have no detectable progeny in the later fetal thymus. In sum, we show that presence of the Lgr5 protein is not a prerequisite for proper thymus organogenesis.
Authors:
Eric Vroegindeweij; Irene van Mourik; Tom Cupedo; Jan J Cornelissen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-03-21
Journal Detail:
Title:  European journal of immunology     Volume:  43     ISSN:  1521-4141     ISO Abbreviation:  Eur. J. Immunol.     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-04-25     Completed Date:  2013-06-17     Revised Date:  2013-10-29    
Medline Journal Info:
Nlm Unique ID:  1273201     Medline TA:  Eur J Immunol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1243-51     Citation Subset:  IM    
Copyright Information:
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
Department of Hematology, Erasmus University Medical Center, Rotterdam, the Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Lineage / immunology
Cell Tracking
Embryo, Mammalian
Epithelial Cells / cytology*,  metabolism
Female
Fetus
Gene Expression
Genes, Reporter
Green Fluorescent Proteins
Mice
Mice, Transgenic
Organogenesis / genetics*,  immunology
Pregnancy
Receptors, G-Protein-Coupled / genetics*,  immunology
Thymus Gland / cytology*,  metabolism
Chemical
Reg. No./Substance:
0/Lgr5 protein, mouse; 0/Receptors, G-Protein-Coupled; 147336-22-9/Green Fluorescent Proteins

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