Document Detail

Characterization of Lewis lung clonal variants in a model of syngeneic pulmonary murine metastases.
MedLine Citation:
PMID:  15387377     Owner:  NLM     Status:  MEDLINE    
Lung cancer is the leading cause of cancer-related mortality world-wide. Since the majority of cancer deaths result from metastatic complications, understanding cellular alterations contributing to organ specific metastases is a continuing cancer research goal. Desirable models involve easy, efficient methodologies for development of pulmonary metastases utilizing genetically related syngeneic tumor cell lines varying in clonogenic frequency and growth rate for comparative studies. This work focused on development and characterization of primary and metastatic Lewis lung subclones (LLCC3, LLC1, LLCab) in a histocompatible C57B1/6 model. Surgical resection of primary tumors utilizing these cell lines resulted in reliable development of pulmonary metastases (> 90% of injected mice), while tail-vein injection proved sporadic (20% of injected mice). The preliminary analysis of selected cell-surface molecules indicates potential genetic differences that may underlie phenotypic variations. The combination of subcutaneous resection methodology and variant cell lines results in robust metastatic lung cancer for testing potential therapeutic interventions.
Bill T Storey; H Keith Pittman; Joseph F Christian; Carl E Haisch; Kathryn M Verbanac
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical & experimental metastasis     Volume:  21     ISSN:  0262-0898     ISO Abbreviation:  Clin. Exp. Metastasis     Publication Date:  2004  
Date Detail:
Created Date:  2004-09-24     Completed Date:  2004-11-29     Revised Date:  2005-03-24    
Medline Journal Info:
Nlm Unique ID:  8409970     Medline TA:  Clin Exp Metastasis     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  265-73     Citation Subset:  IM    
Department of Pathology and Laboratory Medicine, Brown University School of Medicine and Division of Medical Oncology, Rhode Island Hospital, Providence, Rhode Island 02903, USA.
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MeSH Terms
Carcinoma, Lewis Lung / pathology*
Flow Cytometry
Mice, Inbred C57BL
Erratum In:
Clin Exp Metastasis. 2004;21(7):663

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