| Characterization of Legionella Lipopolysaccharide. | |
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MedLine Citation:
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PMID: 23150410 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The lipopolysaccharide(LPS) of Legionella spp. is an immuno-dominant antigen and the basis for Legionella pneumophila serogroup classification. The LPS shows a peculiar structure composed of a very hydrophobic lipid A acylated by long chain fatty acids and an O-antigen-specific chain consisting of homopolymeric legionaminic acid. In this chapter we describe a method for the isolation of LPS from L. pneumophila. In the first part we describe the chemical purification, in the second part we outline the application of monoclonal antibody (mAb) in Western blot and immuno-localization by indirect immunofluorescence. This report does not describe physico-chemical methods that analyze the structure of lipopolysaccharide entities. |
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Authors:
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Christian Lück; Jürgen H Helbig |
Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 954 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-11-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: - |
Other Details:
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Languages: ENG Pagination: 381-390 Citation Subset: - |
Affiliation:
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Institute for Medical Microbiology and Hygiene, University of Technology, Dresden, Germany, Christian.Lueck@tu-dresden.de. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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