Document Detail

Characterization of IK cytokine expression in mouse endometrium during early pregnancy and its significance on implantation.
MedLine Citation:
PMID:  22692555     Owner:  NLM     Status:  Publisher    
The expression of IK cytokine was investigated in the mouse endometrium during early pregnancy (D1-D7 of pregnancy) and pseudopregnancy using real-time PCR, western blotting and immunohistochemical analysis, and the effects of IK cytokine on embryo implantation were observed by injection with antisense IK cytokine oligodeoxynucleotides in the uterine horn. Our data showed that the expression of IK cytokine mRNA increased gradually from D1 to D4 of pregnancy and reached a peak level at D4 of pregnancy (P<0.05). Western blotting and immunohistochemical analysis revealed that the expression of IK cytokine protein increased gradually from D1 to D5 of pregnancy and reached a peak level at D5 of pregnancy (P<0.05). The expression of IK cytokine in the pseudopregnant uterus was significantly lower compared to that in the normal pregnant uterus and the level of the protein never showed a high peak during the whole pseudopregnancy. The expression of IK cytokine at the implantation site was much stronger than that in the peri-implantation site on Day 5 of pregnancy. After 24 and 48 h of injection with antisense IK cytokine oligodexynucleotides in the uterine horn on D3 of pregnancy (i.e. implantation window), the expression of IK cytokine in the uterus was remarkably inhibited, while the expression of major histocompatibility complex Ⅱ (MHCⅡ) increased and the number of implanted embryos significantly decreased in the site of uterine horns receiving antisense IK cytokine (P<0.05). These results suggested that IK cytokine may play a crucial role in implantation.
Ruyue Shao; Xueqing Liu; Yubin Ding; Xuemei Chen; Rufei Gao; Junlin He; Yingxiong Wang
Related Documents :
22374755 - Serotonergic effects on feeding, but not hypothalamus-pituitary-adrenal secretion, are ...
4005395 - Effects of ibuprofen on the disposition kinetics of phenytoin in pregnant rats.
25317815 - Peripheral neuropathy in obstetrics: efficacy and safety of α-lipoic acid supplementati...
25056105 - In vitro assessment of mouse fetal abdominal aortic vascular function.
18446655 - The role of continuous fetal monitoring in the management of preterm premature rupture ...
17850995 - Influence of oxidative stress and metabolic adaptation on pon1 activity and mda level i...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-11
Journal Detail:
Title:  International journal of molecular medicine     Volume:  -     ISSN:  1791-244X     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9810955     Medline TA:  Int J Mol Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Laboratory of Reproductive Biology, Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Altered resting state functional connectivity patterns of the anterior prefrontal cortex in obsessiv...
Next Document:  Correlation of polymer-like solution behaviors with electrospun fiber formation of hydroxypropyl-?-c...