Document Detail


Characterization of a GM7 glioblastoma cell line showing CD133 positivity and both cytoplasmic and nuclear localization of nestin.
MedLine Citation:
PMID:  19082452     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A newly established GM7 cell line was derived from the tumor tissue of a 65-year-old man surgically treated for a relapse of glioblastoma multiforme that occurred 10 months after first surgery following radiotherapy. GM7 cells exhibit spindle or glia-like morphology, and multinucleated giant cells are also present in the culture. The cells proliferate rapidly (PDT is about 18 h) and tend to grow in multilayer without contact inhibition. Using G-banding and SKY, the GM7 cell line was identified as near-triploid with a large number of structural and numerical abnormalities. Repeated karyotyping during long-term cultivation confirmed a chromosome number of 70+/-3 chromosomes per cell. Special attention was paid to the immunocytochemical analysis of protein markers in this cell line; GM7 cells showed strong positivity for CD133, vimentin, nestin, NF-160 and S-100 protein and weak positivity for GFAP and NSE, but were negative for synaptophysin. The most important features of the GM7 cell line are its stable phenotype CD133+/nestin+, which are accepted as stem cell markers in neural stem/progenitor cells, and especially unusual intracellular localization of the IF protein nestin, which was detected and repeatedly confirmed both in the cytoplasm and cell nucleus. For this reason, the new GM7 glioblastoma cell line represents an important model suitable not only for further studies on glioblastoma biology and cancer stem cells, but particularly for the detailed investigation of the role of nestin in transformed cells.
Authors:
Tomas Loja; Petr Chlapek; Petr Kuglik; Martina Pesakova; Alexandra Oltova; Pavel Cejpek; Renata Veselska
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncology reports     Volume:  21     ISSN:  1021-335X     ISO Abbreviation:  Oncol. Rep.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-16     Completed Date:  2009-03-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9422756     Medline TA:  Oncol Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  119-27     Citation Subset:  IM    
Affiliation:
Laboratory of Tumor Biology and Genetics, Institute of Experimental Biology, School of Science, Masaryk University, 611 37 Brno, Czech Republic.
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MeSH Terms
Descriptor/Qualifier:
Aged
Antigens, CD / biosynthesis*
Blotting, Western
Cell Line, Tumor / physiology*,  ultrastructure*
Cell Nucleus / chemistry,  metabolism
Chromosome Aberrations
Cytoplasm / chemistry,  metabolism
Flow Cytometry
Fluorescent Antibody Technique
Glioblastoma / genetics,  metabolism*,  ultrastructure
Glycoproteins / biosynthesis*
Humans
Immunohistochemistry
Intermediate Filament Proteins / metabolism*
Male
Microscopy, Electron, Transmission
Nerve Tissue Proteins / metabolism*
Peptides
Tumor Markers, Biological / analysis
Chemical
Reg. No./Substance:
0/AC133 antigen; 0/Antigens, CD; 0/Glycoproteins; 0/Intermediate Filament Proteins; 0/Nerve Tissue Proteins; 0/Peptides; 0/Tumor Markers, Biological; 0/nestin

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