Document Detail


Characterization of GABA(B) receptors involved in inhibition of motility associated with acetylcholine release in the dog small intestine: possible existence of a heterodimer of GABA(B1) and GABA(B2) subunits.
MedLine Citation:
PMID:  15107576     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Characterization of the gamma-aminobutyric acid (GABA)(B) receptor involved in the motility of dog small intestine was analyzed by application of the microdialysis method to the small intestine of the whole body of the dog. The reverse transcription-polymerase chain reaction (RT-PCR) was used. Intraarterial administration of muscimol induced acceleration of motility associated with acetylcholine (ACh) release, these responses being antagonized by bicuculline. Intraarterial administration of baclofen induced inhibition of motility associated with ACh release, these responses being antagonized by CGP62349. GABA induced inhibition of motility associated with decrease in ACh release. CGP62349 alone induced acceleration of motility associated with increase in ACh release. RT-PCR revealed the presence of mRNAs for both subunits of GABA(B) receptor, GABA(B1) and GABA(B2), in the dog small intestine, although GABA(B1) subunits were 6 isoforms of GABA(B1) (GABA(B1(a)) - GABA(B1(g))), except GABA(B1(d)). Thus, the GABA(B) receptor located at cholinergic neurons as a heterodimer with subunits of GABA(B1) and GABA(B2) in the dog small intestine operates predominantly relative to the GABA(A) receptor in physiological motility.
Authors:
Shunsuke Kawakami; Yasuhito Uezono; Noriaki Makimoto; Akihito Enjoji; Muneshige Kaibara; Takashi Kanematsu; Kohtaro Taniyama
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pharmacological sciences     Volume:  94     ISSN:  1347-8613     ISO Abbreviation:  J. Pharmacol. Sci.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-04-26     Completed Date:  2004-11-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101167001     Medline TA:  J Pharmacol Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  368-75     Citation Subset:  IM    
Affiliation:
Department of Translational Medical Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
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MeSH Terms
Descriptor/Qualifier:
Acetylcholine / metabolism*
Animals
Baclofen / pharmacology
Benzoates / pharmacology
Dimerization
Dogs
Female
GABA Agonists / pharmacology
GABA Antagonists / pharmacology
Gastrointestinal Motility / drug effects*
Intestine, Small / drug effects*,  metabolism
Male
Microdialysis*
Muscimol / pharmacology
Organophosphorus Compounds / pharmacology
RNA, Messenger / analysis,  metabolism
Receptors, GABA-B / analysis,  genetics,  metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/Benzoates; 0/CGP 62349; 0/GABA Agonists; 0/GABA Antagonists; 0/Organophosphorus Compounds; 0/RNA, Messenger; 0/Receptors, GABA-B; 1134-47-0/Baclofen; 2763-96-4/Muscimol; 51-84-3/Acetylcholine
Comments/Corrections
Erratum In:
J Pharmacol Sci. 2005 Dec;99(4):420

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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