Document Detail


Characterization of Fonsecaea pedrosoi melanin.
MedLine Citation:
PMID:  1856679     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The constituents of the melanin complex from mycelial forms of Fonsecaea pedrosoi were partially characterized. The pigment was mainly accumulated on large alkali-extractable, electron-dense cytoplasmic bodies (melanosomes) and, apparently, on the outer layer of the cell wall as external deposits within verrucose outgrowths. Using electron microscopy and Thiéry's periodate/thiosemicarbazide/silver proteinate staining method, glycogen-like particles were also detected at the periphery of the cells. Melanin constituents comprised aromatic and aliphatic/glycosidic structures with a predominance of the latter. Infrared spectra showed the presence of hydroxyl, carbonyl and carboxyl groups. The aliphatic/glycosidic moiety consisted of fatty acids and polysaccharides with protein, in a ratio protein/polysaccharide 1:15. Rhamnose, mannose, galactose and glucose (in the ratio 1:2:4:3.5) were the constituents of the polysaccharide. Lipid components included even-numbered, saturated and unsaturated fatty acids (in the ratio 2:1) ranging from C16 to C18. Palmitic and oleic acids were the prominent fatty acids. Aspartic and glutamic acids, leucine, glycine and alanine were the major amino acids. Non-pigmented cells of F. pedrosoi were studied for comparison with the pigmented forms: they did not accumulate acid-insoluble precursors of melanin.
Authors:
C S Alviano; S R Farbiarz; W De Souza; J Angluster; L R Travassos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of general microbiology     Volume:  137     ISSN:  0022-1287     ISO Abbreviation:  J. Gen. Microbiol.     Publication Date:  1991 Apr 
Date Detail:
Created Date:  1991-08-29     Completed Date:  1991-08-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375371     Medline TA:  J Gen Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  837-44     Citation Subset:  IM    
Affiliation:
Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Ilha, do Fundão, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Carbohydrates / analysis
Chromoblastomycosis / microbiology*
Fatty Acids / analysis
Humans
Melanins / chemistry,  metabolism*
Microscopy, Electron
Mitosporic Fungi / metabolism*,  ultrastructure
Spectrophotometry, Infrared
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Carbohydrates; 0/Fatty Acids; 0/Melanins

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