Document Detail


Characterization of Fe2+-activated acid phosphatase in rat epidermis.
MedLine Citation:
PMID:  3931969     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A particulate acid phosphatase (EC 3.1.3.2, orthophosphoric monoester phosphohydrolase (acid optimum)) was extracted in 1 M KCl, from 2-day rat epidermis. The enzyme has a Mr of 32,000, but two forms, F1 and F2 with pI values of 8.6 and 8.3, respectively, were identified while the pI values of other acid phosphatases soluble in sucrose and Triton X-100 were all acidic. F1 and F2 also differed from other epidermal acid phosphatases because they were (a) activated by Fe2+ and reducing agents, (b) showed immunological cross-reactivity with purple acid phosphatase of rat spleen and (c) dephosphorylated phosvitin and alpha-casein even though they had rather high Km values.
Authors:
A Hara; T Kato; H Sawada; K Fukuyama; W L Epstein
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Comparative biochemistry and physiology. B, Comparative biochemistry     Volume:  82     ISSN:  0305-0491     ISO Abbreviation:  Comp. Biochem. Physiol., B     Publication Date:  1985  
Date Detail:
Created Date:  1985-11-27     Completed Date:  1985-11-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2984730R     Medline TA:  Comp Biochem Physiol B     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  269-74     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / isolation & purification,  metabolism*
Animals
Animals, Newborn
Ascorbic Acid / pharmacology
Enzyme Activation
Female
Immunodiffusion
Iron / pharmacology*
Kinetics
Male
Rats
Rats, Inbred Strains
Skin / enzymology*
Substrate Specificity
Grant Support
ID/Acronym/Agency:
AM 12433/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
50-81-7/Ascorbic Acid; 7439-89-6/Iron; EC 3.1.3.2/Acid Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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