Document Detail


Characterization of FcR Ig-binding sites and epitope mapping.
MedLine Citation:
PMID:  7520815     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The low-affinity receptor for IgG, Fc gamma RII, and the high-affinity receptor for IgE, Fc epsilon RI, are functionally distinct but structurally homologous receptors. These characteristics have been exploited using a chimeric receptor strategy to examine segments of human Fc gamma RII for IgG-binding function. A series of chimeric receptors was generated by exchanging coding regions of the extracellular ligand-binding regions between Fc gamma RII and the Fc epsilon RI alpha chain using splice overlap extension by the polymerase chain reaction. The expression of these chimeric receptors in COS-7 cells and analysis of their IgG/IgE binding capacities have enabled the Ig-binding region of Fc gamma RII to be localized to a subregion of the second extracellular domain. The localization of the Ig-binding region of Fc gamma RII has provided the opportunity of performing site-directed mutagenesis to determine the key amino acids involved in the interaction of the receptor with IgG. These findings demonstrate that the chimeric receptor approach is a powerful technique for the dissection of structure/function relationships of structurally related yet functionally different molecules.
Authors:
P M Hogarth; F L Ierino; M D Hulett
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  ImmunoMethods     Volume:  4     ISSN:  1058-6687     ISO Abbreviation:  Immunomethods     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-09-29     Completed Date:  1994-09-29     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  9306032     Medline TA:  Immunomethods     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  17-24     Citation Subset:  IM    
Affiliation:
Austin Research Institute, Austin Hospital, Heidelberg, Victoria, Australia.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antibodies, Monoclonal
Base Sequence
Binding Sites
Cell Line
Epitopes / analysis*
Fluorescent Antibody Technique
Humans
Immunoglobulin G / metabolism*
Mice
Molecular Sequence Data
Oligonucleotides / chemistry
Receptors, IgE / genetics,  metabolism
Receptors, IgG / genetics,  metabolism*
Recombinant Fusion Proteins / genetics,  metabolism
Rosette Formation
Transfection
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Epitopes; 0/Immunoglobulin G; 0/Oligonucleotides; 0/Receptors, IgE; 0/Receptors, IgG; 0/Recombinant Fusion Proteins

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