Document Detail

Characterization of Fatty Acid biosynthesis in isolated pea root plastids.
MedLine Citation:
PMID:  16668228     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Fatty acid biosynthesis from Na[1-(14)C]acetate was characterized in plastids isolated from primary roots of 7-day-old germinating pea (Pisum sativum L.) seeds. Fatty acid synthesis was maximum at 82 nanomoles per hour per milligram protein in the presence of 200 micromolar acetate, 0.5 millimolar each of NADH, NADPH, and coenzyme A, 6 millimolar each of ATP and MgCl(2), 1 millimolar each of MnCl(2) and glycerol-3-phosphate, 15 millimolar KHCO(3), 0.31 molar sucrose, and 0.1 molar Bis-Tris-propane, pH 8.0, incubated at 35 degrees C. At the standard incubation temperature of 25 degrees C, fatty acid synthesis was essentially linear for up to 6 hours with 80 to 120 micrograms per milliliter plastid protein. ATP and coenzyme A were absolute requirements, whereas divalent cations, potassium bicarbonate, and reduced nucleotides all variously improved activity two- to 10-fold. Mg(2+) and NADH were the preferred cation and nucleotide, respectively. Glycerol-3-phosphate had little effect, whereas dithiothreitol and detergents generally inhibited the incorporation of [(14)C]acetate into fatty acids. On the average, the principal radioactive products of fatty acid biosynthesis were approximately 39% palmitic, 9% stearic, and 52% oleic acid. The proportions of these fatty acids synthesized depended on the experimental conditions.
R J Stahl; S A Sparace
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant physiology     Volume:  96     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1991 Jun 
Date Detail:
Created Date:  2010-06-29     Completed Date:  2010-06-29     Revised Date:  2010-09-15    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  602-8     Citation Subset:  -    
Plant Science Department, Macdonald College of McGill University, 21111 Lakeshore Road, Ste. Anne de Bellevue, Quebec, H9X 1CO, Canada.
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